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Hybrid between Danio rerio female and Danio nigrofasciatus male produces aneuploid sperm with limited fertilization capacity

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Title: Hybrid between Danio rerio female and Danio nigrofasciatus male produces aneuploid sperm with limited fertilization capacity
Authors: Endoh, Mitsuru Browse this author
Shima, Fumika Browse this author
Havelka, Milos Browse this author
Asanuma, Rei Browse this author
Yamaha, Etsuro Browse this author →KAKEN DB
Fujimoto, Takafumi Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Issue Date: 29-May-2020
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 15
Issue: 5
Start Page: e0233885
Publisher DOI: 10.1371/journal.pone.0233885
Abstract: In the Danio species, interspecific hybridization has been conducted in several combinations. Among them, only the hybrid between a zebrafish (D. rerio) female and a spotted danio (D. nigrofasciatus) male was reported to be fertile. However, beyond these investigations, by means of reproductive biology, gametes of the hybrid have also not been investigated genetically. For this study, we induced a hybrid of the D. rerio female and D. nigrofasciatus male in order to study its developmental capacity, reproductive performance and gametic characteristics. Its hybrid nature was genetically verified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the rhodopsin gene. Almost all the hybrids (36/37) were males, and only one was female. Developing oocytes were observed in the hybrid female, but ovulated eggs have not been obtained thus far. Microscopic observation revealed various head sizes of sperm in the hybrid males. Flow cytometry showed that the hybrid males generated aneuploid sperm with various ploidy levels up to diploidy. In backcrosses between D. rerio females and hybrid males, fertilization rates were significantly lower than the control D. rerio, and most resultant progeny with abnormal appearance exhibited various kinds of aneuploidies ranging from haploidy to triploidy, but only one viable progeny, which survived more than four months, was triploid. This suggested the contribution of fertile diploid sperm of the hybrid male to successful fertilization and development.
Type: article
Appears in Collections:高等教育推進機構 (Institute for the Advancement of Higher Education) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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