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Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method

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Title: Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method
Authors: Thapa, Jeewan Browse this author
Maharjan, Bhagwan Browse this author
Malla, Meena Browse this author
Fukushima, Yukari Browse this author
Poudel, Ajay Browse this author
Pandey, Basu Dev Browse this author
Hyashida, Kyoko Browse this author
Gordon, Stephen V. Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Loop mediated isothermal amplification (LAMP)
Methyl green
Mycobacterium tuberculosis
Tuberculosis
Issue Date: Jul-2019
Publisher: Elsevier
Journal Title: Tuberculosis
Volume: 117
Start Page: 1
End Page: 6
Publisher DOI: 10.1016/j.tube.2019.05.004
PMID: 31378262
Abstract: The purpose of this study was to develop a simple visual methyl green (MeG) based dry loop-mediated isothermal amplification (LAMP) method for early detection of Mycobacterium tuberculosis (MTB) from clinical samples. We identified MeG as an indicator of a positive LAMP reaction, where a positive reaction gave a blue-green color while a negative reaction was colorless. The MeG MTB-LAMP system was further simplified by drying all reagents for ease of use, and was then validated for its ability to diagnose TB directly using Nepalese clinical samples. We evaluated the dry MeG MTB-LAMP with 69 new TB suspected samples from patients that did not have a confirmed history of TB treatment and found the sensitivity in culture positive samples as 92.8% (13/14) and specificity in culture negative samples as 96.3% (53/55). Our LAMP system has the potential to be a point of care test for early diagnosis of active TB in developing countries.
Rights: ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/79002
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: THAPA JEEWAN

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