|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Direct detection of Mycobacterium tuberculosis in clinical samples by a dry methyl green loop-mediated isothermal amplification (LAMP) method |
| Authors: | Thapa, Jeewan Browse this author | | Maharjan, Bhagwan Browse this author | | Malla, Meena Browse this author | | Fukushima, Yukari Browse this author | | Poudel, Ajay Browse this author | | Pandey, Basu Dev Browse this author | | Hyashida, Kyoko Browse this author | | Gordon, Stephen V. Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Loop mediated isothermal amplification (LAMP) | | Methyl green | | Mycobacterium tuberculosis | | Tuberculosis |
| Issue Date: | Jul-2019 |
| Publisher: | Elsevier |
| Journal Title: | Tuberculosis |
| Volume: | 117 |
| Start Page: | 1 |
| End Page: | 6 |
| Publisher DOI: | 10.1016/j.tube.2019.05.004 |
| PMID: | 31378262 |
| Abstract: | The purpose of this study was to develop a simple visual methyl green (MeG) based dry loop-mediated isothermal amplification (LAMP) method for early detection of Mycobacterium tuberculosis (MTB) from clinical samples. We identified MeG as an indicator of a positive LAMP reaction, where a positive reaction gave a blue-green color while a negative reaction was colorless. The MeG MTB-LAMP system was further simplified by drying all reagents for ease of use, and was then validated for its ability to diagnose TB directly using Nepalese clinical samples. We evaluated the dry MeG MTB-LAMP with 69 new TB suspected samples from patients that did not have a confirmed history of TB treatment and found the sensitivity in culture positive samples as 92.8% (13/14) and specificity in culture negative samples as 96.3% (53/55). Our LAMP system has the potential to be a point of care test for early diagnosis of active TB in developing countries. |
| Rights: | ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79002 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: THAPA JEEWAN
|
