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Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source

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Title: Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source
Authors: Yamaguchi, Takahiro Browse this author
Narsico, Joemark Browse this author
Kobayashi, Takanori Browse this author
Inoue, Akira Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Keywords: Poly(3-hydroyxybutylate)
Alginate
Alginate-assimilating gene cluster
Poly(3-hydroyxybutylate)-synthesis gene cluster
Hydrogenophaga sp. UMI-18
Issue Date: Aug-2019
Publisher: The Society for Biotechnology, Japan
Elsevier
Journal Title: Journal of bioscience and bioengineering
Volume: 128
Issue: 2
Start Page: 203
End Page: 208
Publisher DOI: 10.1016/j.jbiosc.2019.02.008
Abstract: A novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 that produces poly(3-hydroyxybutylate) (PHB) in the alginate-mineral salt (AMS) medium containing 1% (w/v) sodium alginate as a sole carbon source was isolated from a decayed brown seaweed litter. The yield of PHB produced by strain UMI-18 was 1.1 +/- 0.15 g/L of AMS and the PHB content in dried cell pellet was 58 +/- 4% (w/w). Glucose, fructose, galactose, mannose, mannitol, sucrose and lactose were also available for the production of PHB by strain UMI-18. The yield of PHB in 1% (w/v) carbohydrate media reached 2.03-2.24 g/L for glucose and fructose, 0.75-1.64 g/L for alginate, galactose, mannitol and sucrose, and similar to 0.15 g/L for lactose. The PHB produced by strain UMI-18 showed a glass-transition temperature (T-g) at 4 degrees C, a melting temperature at 175 degrees C, and an average molecular mass of 860 kDa. Draft genome analysis of the strain UMI-18 revealed that an alginate-assimilating gene cluster is located in contig 8 comprising 453,520 bp and the PHB-synthesis gene cluster is in contig 15 comprising 653,793 bp. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/79013
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 尾島 孝男

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