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Isoflurane induces c-Fos expression in the area postrema of the rat

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Title: Isoflurane induces c-Fos expression in the area postrema of the rat
Authors: Hase, Tetsutaro Browse this author
Hashimoto, Toshikazu Browse this author →KAKEN DB
Saito, Hitoshi Browse this author
Uchida, Yosuke Browse this author
Kato, Rui Browse this author →KAKEN DB
Tsuruga, Kenkichi Browse this author →KAKEN DB
Takita, Koichi Browse this author →KAKEN DB
Morimoto, Yuji Browse this author →KAKEN DB
Keywords: Isoflurane
Area postrema
Issue Date: Aug-2019
Publisher: Springer
Journal Title: Journal of anesthesia
Volume: 33
Issue: 4
Start Page: 562
End Page: 566
Publisher DOI: 10.1007/s00540-019-02662-0
PMID: 31317263
Abstract: IntroductionVolatile anesthetics are speculated to cause postoperative nausea and vomiting via stimulation of the chemoreceptor trigger zone (CTZ). However, the precise mechanism underlying the emetic action of these drugs is not well understood. In this study, we assessed whether isoflurane induced the expression of c-Fos, a neuronal activation marker, in the area postrema (AP), the locus of the CTZ, in rats, which do not have vomiting action.Materials and methodsMale rats were exposed to 1.3% isoflurane for 0-240min, or to various concentrations of isoflurane (0, 1.3%, or 2.6%) for 120min. Finally, the rats were exposed to 1.3% isoflurane for 120min after ondansetron administration. After the treatments, immunohistochemistry of the rat AP was performed using c-Fos antibody staining.ResultsOne-way analysis of variance showed that isoflurane exposure significantly increased c-Fos expression in the AP; however, the rats pretreated with 4mg/kg ondansetron showed significantly decreased c-Fos expression. Moreover, we evaluated the effect of the anesthetic on inducing pica in the rats, and found that kaolin intake was not influenced by isoflurane exposure.ConclusionOverall, these results suggest that isoflurane activates AP neurons and may be involved in the emetic mechanism of isoflurane. This study further suggests the feasibility of using rats as a model for studying emetic mechanisms of drugs, despite their lack of vomit action.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷 徹太郎

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