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Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8(+) T Cells among Vaccinated and Unvaccinated Cattle in Malawi

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Title: Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8(+) T Cells among Vaccinated and Unvaccinated Cattle in Malawi
Authors: Chatanga, Elisha Browse this author
Hayashida, Kyoko Browse this author
Muleya, Walter Browse this author
Kusakisako, Kodai Browse this author
Moustafa, Mohamed Abdallah Mohamed Browse this author
Salim, Bashir Browse this author
Katakura, Ken Browse this author
Sugimoto, Chihiro Browse this author
Nonaka, Nariaki Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Keywords: Malawi
Theileria parva
genetic diversity
vaccine
Muguga cocktail
Issue Date: May-2020
Publisher: MDPI
Journal Title: Pathogens
Volume: 9
Issue: 5
Start Page: 334
Publisher DOI: 10.3390/pathogens9050334
Abstract: East Coast fever (ECF) is an acute fatal tick-borne disease of cattle caused by Theileria parva. It causes major losses in exotic and crossbreed cattle, but this could be prevented by a vaccine of T. parva if the vaccine is selected properly based on information from molecular epidemiology studies. The Muguga cocktail (MC) vaccine (Muguga, Kiambu 5 and Serengeti-transformed strains) has been used on exotic and crossbreed cattle. A total of 254 T. parva samples from vaccinated and unvaccinated cattle were used to understand the genetic diversity of T. parva in Malawi using partial sequences of the Tp1 and Tp2 genes encoding T. parva CD8(+) antigens, known to be immunodominant and current candidate antigens for a subunit vaccine. Single nucleotide polymorphisms were observed at 14 positions (3.65%) in Tp1 and 156 positions (33.12%) in Tp2, plus short deletions in Tp1, resulting in 6 and 10 amino acid variants in the Tp1 and Tp2 genes, respectively. Most sequences were either identical or similar to T. parva Muguga and Kiambu 5 strains. This may suggest the possible expansion of vaccine components into unvaccinated cattle, or that a very similar genotype already existed in Malawi. This study provides information that support the use of MC to control ECF in Malawi.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/79032
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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