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Phosphorylated chitin increased bone formation when implanted into rat calvaria with the Ti-device

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Biomed. Mater. Eng. 31(1) 47-57.pdf437.72 kBPDFView/Open
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Title: Phosphorylated chitin increased bone formation when implanted into rat calvaria with the Ti-device
Authors: Song, Qin Browse this author
Yagami, Kimitoshi Browse this author
Furusawa, Toshitake Browse this author
Takita, Hiroko Browse this author →KAKEN DB
Kurasaki, Masaaki Browse this author →KAKEN DB
Tokura, Seiichi Browse this author →KAKEN DB
Morimoto, Koichi Browse this author
Sammons, Rachel Browse this author
Iku, Shouhei Browse this author
Kuboki, Yoshinori Browse this author →KAKEN DB
Keywords: Chitin
enhanced bone formation
Issue Date: 12-May-2020
Publisher: IOS Press
Journal Title: Bio-medical materials and engineering
Volume: 31
Issue: 1
Start Page: 47
End Page: 57
Publisher DOI: 10.3233/BME-201079
Abstract: BACKGROUND: Previously we found that a group of phosphorylated proteins (SIBLINGs) in bone binds with the Ti-device, and increases the early bone formation around the Ti-implants remarkably. From these results, we explained the biochemical mechanism of a strong bond between living bone and Ti, which was discovered by Branemark and colleagues. For the clinical application of our findings, we need a large amount of these proteins or their substitutes. OBJECTIVE: We aimed to create a new molecule that equips with essential functions of SIBLINGs, Ti-binding, and bone enhancement around the Ti implant. METHODS: We chemically phosphorylated chitin and obtained a soluble form of phosphorylated chitin (P-chitin). In this solution, we immersed the Ti-devices of web-form (TW) which we previously developed and obtained the P-chitin coated TWs. Then we tested the P-chitin coated TWs for their calcification ability in vitro, and bone enhancing ability in vivo, by implanting them into rat calvaria. We compared the P-chitin coated TW and the non-coated TW in regard to their calcification and bone enhancing abilities. RESULTS: Ti-devices coated with phosphorylated-chitin induced a ten times higher calcification in vitro at 20 days, and four times more elevated amount of bone formation in vivo at two weeks than the uncoated Ti-device. CONCLUSIONS: Phosphorylated chitin could be a partial substitute of bone SIBLING proteins and are clinically applicable to accelerate bone formation around the Ti implants, thereby achieving the strong bond between living bone and Ti.
Rights: The final publication is available at IOS Press through
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保木 芳徳

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