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Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure
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Title: | Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure |
Authors: | Aly, Mahmoud Khamis Browse this author | Ninomiya, Kensuke Browse this author →KAKEN DB | Adachi, Shungo Browse this author | Natsume, Tohru Browse this author | Hirose, Tetsuro Browse this author →KAKEN DB |
Keywords: | Nuclear stress bodies | Long noncoding RNA | HSATIII | RNA-binding protein | Primate-specific genes | Thermal stress |
Issue Date: | 20-Aug-2019 |
Publisher: | Elsevier |
Journal Title: | Biochemical and biophysical research communications |
Volume: | 516 |
Issue: | 2 |
Start Page: | 419 |
End Page: | 423 |
Publisher DOI: | 10.1016/j.bbrc.2019.06.061 |
PMID: | 31227213 |
Abstract: | Nuclear stress bodies (nSBs) are thermal stress-inducible membrane-less nuclear bodies that are formed on highly repetitive satellite Ill architectural noncoding RNAs (HSATIII arcRNAs). Upon thermal stress exposure, HSATIII expression is induced to sequestrate specific sets of RNA-binding proteins and form nSBs. The major population of nSBs contain SAFB as a marker, whereas the minor population are SAFB-negative. Here, we found that HNRNPM, which was previously reported to localize in nuclear foci adjacent to SAFB-positive foci upon thermal stress, localizes in a minor population of HSATIII-dependent nSBs. Hence, we used the terms nSB-S and nSB-M to distinguish the SAFB foci and HNRNPM foci, respectively. Analysis of the components of the nSBs revealed that each set contains distinct RNA-binding proteins, including SLTM and NCO5A in nSB-Ss and HNRNPA1 and HNRNPH1 in nSB-Ms. Overall, our findings indicate that two sets of nSBs containing HSATIII arcRNAs and distinct sets of RNA-binding proteins are formed upon thermal stress exposure. |
Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/79081 |
Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 廣瀬 哲郎
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