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Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/79081

Title: Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure
Authors: Aly, Mahmoud Khamis Browse this author
Ninomiya, Kensuke Browse this author →KAKEN DB
Adachi, Shungo Browse this author
Natsume, Tohru Browse this author
Hirose, Tetsuro Browse this author →KAKEN DB
Keywords: Nuclear stress bodies
Long noncoding RNA
HSATIII
RNA-binding protein
Primate-specific genes
Thermal stress
Issue Date: 20-Aug-2019
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 516
Issue: 2
Start Page: 419
End Page: 423
Publisher DOI: 10.1016/j.bbrc.2019.06.061
PMID: 31227213
Abstract: Nuclear stress bodies (nSBs) are thermal stress-inducible membrane-less nuclear bodies that are formed on highly repetitive satellite Ill architectural noncoding RNAs (HSATIII arcRNAs). Upon thermal stress exposure, HSATIII expression is induced to sequestrate specific sets of RNA-binding proteins and form nSBs. The major population of nSBs contain SAFB as a marker, whereas the minor population are SAFB-negative. Here, we found that HNRNPM, which was previously reported to localize in nuclear foci adjacent to SAFB-positive foci upon thermal stress, localizes in a minor population of HSATIII-dependent nSBs. Hence, we used the terms nSB-S and nSB-M to distinguish the SAFB foci and HNRNPM foci, respectively. Analysis of the components of the nSBs revealed that each set contains distinct RNA-binding proteins, including SLTM and NCO5A in nSB-Ss and HNRNPA1 and HNRNPH1 in nSB-Ms. Overall, our findings indicate that two sets of nSBs containing HSATIII arcRNAs and distinct sets of RNA-binding proteins are formed upon thermal stress exposure.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/79081
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 廣瀬 哲郎

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