| Title: | Isolation of Candidatus Bartonella rousetti and Other Bat-associated Bartonellae from Bats and Their Flies in Zambia |
| Authors: | Qiu, Yongjin Browse this author |
| Kajihara, Masahiro Browse this author |
| Nakao, Ryo Browse this author |
| Mulenga, Evans Browse this author |
| Harima, Hayato Browse this author |
| Hang'ombe, Bernard Mudenda Browse this author |
| Eto, Yoshiki Browse this author |
| Changula, Katendi Browse this author |
| Mwizabi, Daniel Browse this author |
| Sawa, Hirofumi Browse this author |
| Higashi, Hideaki Browse this author |
| Mweene, Aaron Browse this author |
| Takada, Ayato Browse this author |
| Simuunza, Martin Browse this author |
| Sugimoto, Chihiro Browse this author →KAKEN DB |
| Keywords: | Bartonella |
| bat fly |
| bat |
| PCR |
| isolation |
| Zambia |
| Issue Date: | Jun-2020 |
| Publisher: | MDPI |
| Journal Title: | Pathogens |
| Volume: | 9 |
| Issue: | 6 |
| Start Page: | 469 |
| Publisher DOI: | 10.3390/pathogens9060469 |
| Abstract: | Bat-associated bartonellae, including Bartonella mayotimonensis and Candidatus Bartonella rousetti, were recently identified as emerging and potential zoonotic agents, respectively. However, there is no report of bat-associated bartonellae in Zambia. Thus, we aimed to isolate and characterize Bartonella spp. from bats and bat flies captured in Zambia by culturing and PCR. Overall, Bartonella spp. were isolated from six out of 36 bats (16.7%), while Bartonella DNA was detected in nine out of 19 bat flies (47.3%). Subsequent characterization using a sequence of five different genes revealed that three isolates obtained from Egyptian fruit bats (Rousettus aegyptiacus) were Ca. B. rousetti. The isolates obtained from insectivorous bats (Macronycteris vittatus) were divided into two previously unclassified bat-associated bartonellae. A phylogenetic analysis of the six genotypes of Bartonella gltA sequences from nine pathogen-positive bat flies revealed that three genotypes belonged to the same clades as bat-associated bartonellae, including Ca. B. rousetti. The other three genotypes represented arthropod-associated bartonellae, which have previously been isolated only from ectoparasites. We demonstrated that Ca. B. rousetti is maintained between bats (R. aegyptiacus) and bat flies in Zambia. Continuous surveillance of Bartonella spp. in bats and serological surveys in humans in Africa are warranted to evaluate the public health importance of bat-associated bartonellae. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79174 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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