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Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases

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Title: Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
Authors: Marsela, Megasari Browse this author
Hayashida, Kyoko Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Chatanga, Elisha Browse this author
Gaithuma, Alex Kiarie Browse this author
Naoko, Kawai Browse this author
Musaya, Janelisa Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Yamagishi, Junya Browse this author →KAKEN DB
Keywords: Cattle
Epidemiology
AAT
HAT
Malawi
Trypanosome
Issue Date: 20-Jul-2020
Publisher: EDP Sciences
Journal Title: Parasite
Volume: 27
Start Page: 46
Publisher DOI: 10.1051/parasite/2020043
Abstract: This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detectTrypanosoma brucei rhodesiense. Trypanosoma congolensewas the most prevalentTrypanosomasp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence ofT. b. rhodesiensedetected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes andT.b.rhodesiensefrom cattle at the human-livestock-wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/79179
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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