Hokkaido University Collection of Scholarly and Academic Papers >
Global Institution for Collaborative Research and Education : GI-CoRE >
Peer-reviewed Journal Articles, etc >
Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
This item is licensed under:Creative Commons Attribution 4.0 International
Title: | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
Authors: | Marsela, Megasari Browse this author | Hayashida, Kyoko Browse this author | Nakao, Ryo Browse this author →KAKEN DB | Chatanga, Elisha Browse this author | Gaithuma, Alex Kiarie Browse this author | Naoko, Kawai Browse this author | Musaya, Janelisa Browse this author | Sugimoto, Chihiro Browse this author →KAKEN DB | Yamagishi, Junya Browse this author →KAKEN DB |
Keywords: | Cattle | Epidemiology | AAT | HAT | Malawi | Trypanosome |
Issue Date: | 20-Jul-2020 |
Publisher: | EDP Sciences |
Journal Title: | Parasite |
Volume: | 27 |
Start Page: | 46 |
Publisher DOI: | 10.1051/parasite/2020043 |
Abstract: | This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detectTrypanosoma brucei rhodesiense. Trypanosoma congolensewas the most prevalentTrypanosomasp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence ofT. b. rhodesiensedetected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes andT.b.rhodesiensefrom cattle at the human-livestock-wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/79179 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|