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Blockade of Platelet-Derived Growth Factor Signaling Inhibits Choroidal Neovascularization and Subretinal Fibrosis in Mice
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| Title: | Blockade of Platelet-Derived Growth Factor Signaling Inhibits Choroidal Neovascularization and Subretinal Fibrosis in Mice |
| Authors: | Liu, Ye Browse this author | | Noda, Kousuke Browse this author →KAKEN DB | | Murata, Miyuki Browse this author →KAKEN DB | | Wu, Di Browse this author | | Kanda, Atsuhiro Browse this author →KAKEN DB | | Ishida, Susumu Browse this author →KAKEN DB |
| Keywords: | age-related macular degeneration | | subretinal fibrosis | | platelet derived growth factor receptor-beta | | choroidal neovascularization | | pericyte |
| Issue Date: | Jul-2020 |
| Publisher: | MDPI |
| Journal Title: | Journal of clinical medicine |
| Volume: | 9 |
| Issue: | 7 |
| Start Page: | 2242 |
| Publisher DOI: | 10.3390/jcm9072242 |
| Abstract: | Neovascular age related macular degeneration (nAMD) leads to severe vision loss worldwide and is characterized by the formation of choroidal neovascularization (CNV) and fibrosis. In the current study, we aimed to investigate the effect of blockade for platelet derived growth factor receptor-beta (PDGFR-beta) on the formation of choroidal neovascularization and fibrosis in the laser-induced CNV model in mice. Firstly, the presence of PDGFR-beta in CNV lesions were confirmed. Intravitreal injection of PDGFR-beta neutralizing antibody significantly reduced the size of CNV and subretinal fibrosis. Additionally, subretinal hyperreflective material (SHRM), a landmark feature on OCT as a risk factor for subretinal fibrosis formation in nAMD patients was also suppressed by PDGFR-beta blockade. Furthermore, pericytes were abundantly recruited to the CNV lesions during CNV formation, however, blockade of PDGFR-beta significantly reduced pericyte recruitment. In addition, PDGF-BB stimulation increased the migration of the rat retinal pericyte cell line, R-rPCT1, which was abrogated by the neutralization of PDGFR-beta. These results indicate that blockade of PDGFR-beta attenuates laser-induced CNV and fibrosis through the inhibition of pericyte migration. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79201 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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