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Blockade of Platelet-Derived Growth Factor Signaling Inhibits Choroidal Neovascularization and Subretinal Fibrosis in Mice

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Title: Blockade of Platelet-Derived Growth Factor Signaling Inhibits Choroidal Neovascularization and Subretinal Fibrosis in Mice
Authors: Liu, Ye Browse this author
Noda, Kousuke Browse this author →KAKEN DB
Murata, Miyuki Browse this author →KAKEN DB
Wu, Di Browse this author
Kanda, Atsuhiro Browse this author →KAKEN DB
Ishida, Susumu Browse this author →KAKEN DB
Keywords: age-related macular degeneration
subretinal fibrosis
platelet derived growth factor receptor-beta
choroidal neovascularization
pericyte
Issue Date: Jul-2020
Publisher: MDPI
Journal Title: Journal of clinical medicine
Volume: 9
Issue: 7
Start Page: 2242
Publisher DOI: 10.3390/jcm9072242
Abstract: Neovascular age related macular degeneration (nAMD) leads to severe vision loss worldwide and is characterized by the formation of choroidal neovascularization (CNV) and fibrosis. In the current study, we aimed to investigate the effect of blockade for platelet derived growth factor receptor-beta (PDGFR-beta) on the formation of choroidal neovascularization and fibrosis in the laser-induced CNV model in mice. Firstly, the presence of PDGFR-beta in CNV lesions were confirmed. Intravitreal injection of PDGFR-beta neutralizing antibody significantly reduced the size of CNV and subretinal fibrosis. Additionally, subretinal hyperreflective material (SHRM), a landmark feature on OCT as a risk factor for subretinal fibrosis formation in nAMD patients was also suppressed by PDGFR-beta blockade. Furthermore, pericytes were abundantly recruited to the CNV lesions during CNV formation, however, blockade of PDGFR-beta significantly reduced pericyte recruitment. In addition, PDGF-BB stimulation increased the migration of the rat retinal pericyte cell line, R-rPCT1, which was abrogated by the neutralization of PDGFR-beta. These results indicate that blockade of PDGFR-beta attenuates laser-induced CNV and fibrosis through the inhibition of pericyte migration.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/79201
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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