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FTY720 Protects Against Ischemia–Reperfusion Injury by Preventing the Redistribution of Tight Junction Proteins and Decreases Inflammation in the Subacute Phase in an Experimental Stroke Model

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Title: FTY720 Protects Against Ischemia–Reperfusion Injury by Preventing the Redistribution of Tight Junction Proteins and Decreases Inflammation in the Subacute Phase in an Experimental Stroke Model
Authors: Wang, Zifeng Browse this author
Higashikawa, Kei Browse this author →KAKEN DB
Yasui, Hironobu Browse this author →KAKEN DB
Kuge, Yuji Browse this author →KAKEN DB
Ohno, Yusuke Browse this author →KAKEN DB
Kihara, Akio Browse this author →KAKEN DB
Midori, Yenari A. Browse this author
Houkin, Kiyohiro Browse this author →KAKEN DB
Kawabori, Masahito Browse this author →KAKEN DB
Keywords: FTY720
Sphingosine-1-phosphate receptor
Brain ischemia–reperfusion
Blood–brain barrier
Inflammation
Apoptosis
Issue Date: Oct-2020
Publisher: Springer
Journal Title: Translational Stroke Research
Volume: 11
Start Page: 1103
End Page: 1116
Publisher DOI: 10.1007/s12975-020-00789-x
Abstract: Injury due to brain ischemia followed by reperfusion (I/R) may be an important therapeutic target in the era of thrombectomy. FTY720, a widely known sphingosine-1-phosphate receptor agonist, exerts various neuroprotective effects. The aim of this study was to examine the protective effect of FTY720 with respect to I/R injury, especially focusing on blood?brain barrier (BBB) protection and anti-inflammatory effects. Male rats were subjected to transient ischemia and administered vehicle or 0.5 or 1.5 mg/kg of FTY720 immediately before reperfusion. Positron emission tomography (PET) with [18F]DPA-714 was performed 2 and 9 days after the insult to serially monitor neuroinflammation. Bovine and rat brain microvascular endothelial cells (MVECs) were also subjected to oxygen-glucose deprivation (OGD) and reperfusion, and administered FTY720, phosphorylated-FTY720 (FTY720-P), or their inhibitor. FTY720 dose-dependently reduced cell death, the infarct size, cell death including apoptosis, and inflammation. It also ameliorated BBB disruption and neurological deficits compared to in the vehicle group. PET indicated that FTY720 significantly inhibited the worsening of inflammation in later stages. FTY720-P significantly prevented the intracellular redistribution of tight junction proteins but did not increase their mRNA expression. These results suggest that FTY720 can ameliorate I/R injury by protecting the BBB and regulating neuroinflammation.
Rights: © The Author(s) 2020
http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/79237
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川堀 真人

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