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Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep

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Title: Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep
Authors: Tsuji, Hyuma Browse this author
Otsuka, Ryo Browse this author
Wada, Haruka Browse this author →KAKEN DB
Murata, Tomoki Browse this author
Sasaki, Airi Browse this author
Itoh, Mizuho Browse this author
Baghdadi, Muhammad Browse this author →KAKEN DB
Sasaki, Erika Browse this author
Seino, Ken-ichiro Browse this author →KAKEN DB
Issue Date: 28-Jul-2020
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 10
Issue: 1
Start Page: 12625
Publisher DOI: 10.1038/s41598-020-69690-9
Abstract: Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) as the resource of therapeutic cells/tissue. However, immune suppression is still needed when the donor-recipient combination is allogeneic. We have reported previously that mouse PSCs-derived immunosuppressive cells contribute to prolonged survival of grafts derived from the same mouse PSCs in allogeneic recipients. For its clinical application, a preclinical study using non-human primates such as common marmoset must be performed. In this study, we established the induction protocol of immunosuppressive cells from common marmoset ES cells. Although similar immunosuppressive macrophages could not be induced by same protocol as that for mouse PSCs, we employed an inhibitor for histone methyltransferase, DZNep, and succeeded to induce them. The DZNep-treated macrophage-like cells expressed several immunosuppressive molecules and significantly inhibited allogeneic mixed lymphocyte reaction. The immunosuppressive cells from non-human primate ESCs will help to establish an immunoregulating strategy in regenerative medicine using PSCs.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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