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Volume 68 Number 3 >

Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1)

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.68.3.159

Title: Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1)
Authors: Shehata, Ayman Ahmed Browse this author
Bando, Hironori Browse this author →KAKEN DB
Fukuda, Yasuhiro Browse this author →KAKEN DB
Kabir, Mohammad Hazzaz Bin Browse this author
Murakoshi, Fumi Browse this author
Itoh, Megumi Browse this author
Fujikura, Atsushi Browse this author
Okawa, Hiroaki Browse this author
Takuto, Endo Browse this author
Akira, Goto Browse this author
Kachi, Masayuki Browse this author
Nakayama, Toshie Browse this author
Kano, Yuto Browse this author
Oishi, Shoko Browse this author
Otomaru, Konosuke Browse this author
Kazama, Kei Browse this author
Essa, Mohamed Ibrahim Browse this author
Kato, Kentaro Browse this author
Keywords: Calves
Cryptosporidium
CSpV1
PCR
Issue Date: Aug-2020
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 68
Issue: 3
Start Page: 159
End Page: 170
Abstract: Cryptosporidium is an apicomplexan zoonotic parasite that infects most mammals, including humans. Cryptosporidium parvum virus type 1 (CSpV1) is the first member within the Partitiviridae family recognized to infect protozoan hosts. Cryptosporidium tracking based on CSpV1 detection has been attempted; however, each study used different conditions for the PCR protocol, primers, and target viral sequences. Accordingly, the sensitivity of PCR-based CSpV1 detection remains unclear. In addition, oocyst purification from clinical samples can be problematic due to small number of oocysts, sample degradation and low yield efficiency of currently used purification methods. Here we show that the second half of the coding region of dsRNA2 can be detected from various types of clinical samples, without the need for oocyst purification, by using a semi-nested-PCR technique. Furthermore, we show that the short sequence targeted in this study has higher diversity than the Cryptosporidium GP60 gene. Taken together, our findings suggest that this method could be used as an important tracking marker for Cryptosporidium species.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/79325
Appears in Collections:Japanese Journal of Veterinary Research > Volume 68 Number 3

Submitter: 獣医学部図書室

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