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Surface-Enhanced Infrared Absorption Spectroscopy of Bacterial Nitric Oxide Reductase under Electrochemical Control Using a Vibrational Probe of Carbon Monoxide

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Title: Surface-Enhanced Infrared Absorption Spectroscopy of Bacterial Nitric Oxide Reductase under Electrochemical Control Using a Vibrational Probe of Carbon Monoxide
Authors: Kato, Masaru Browse this author →KAKEN DB
Nakagawa, Shogo Browse this author
Tosha, Takehiko Browse this author →KAKEN DB
Shiro, Yoshitsugu Browse this author →KAKEN DB
Masuda, Yuya Browse this author
Nakata, Kou Browse this author →KAKEN DB
Yagi, Ichizo Browse this author →KAKEN DB
Issue Date: 6-Sep-2018
Publisher: American Chemical Society
Journal Title: The Journal of Physical Chemistry Letters
Volume: 9
Issue: 17
Start Page: 5196
End Page: 5200
Publisher DOI: 10.1021/acs.jpclett.8b02581
Abstract: Nitric oxide reductases (NORs) reduce nitric oxide to nitrous oxide in the denitrification pathway of the global nitrogen cycle. NORs contain four iron cofactors and the NO reduction occurs at the heme b3/nonheme FeB binuclear active site. The determination of reduction potentials of the iron cofactors will help us elucidate the enzymatic reaction mechanism. However, previous reports on these potentials remain controversial. Herein, we performed electrochemical and surface-enhanced infrared absorption (SEIRA) spectroscopic measurements of Pseudomonas aeruginosa NOR immobilized on gold electrodes. Cyclic voltammograms exhibited two reduction peaks at −0.11 and −0.44 V vs SHE, and a SEIRA spectrum using a vibrational probe of CO showed a characteristic band at 1972 cm–1 at −0.4 V vs SHE, which was assigned to νCO of heme b3–CO. Our results suggest that the reduction of heme b3 initiates the enzymatic NO reduction.
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of physical chemistry letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jpclett.8b02581
Type: article (author version)
URI: http://hdl.handle.net/2115/79350
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 優

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