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Transcriptomic analysis under ethylene precursor treatment uncovers the regulation of gene expression linked to sexual reproduction in the dioecious red alga Pyropia pseudolinearis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/79387

Title: Transcriptomic analysis under ethylene precursor treatment uncovers the regulation of gene expression linked to sexual reproduction in the dioecious red alga Pyropia pseudolinearis
Authors: Yanagisawa, Ryo Browse this author
Sekine, Naoki Browse this author
Mizuta, Hiroyuki Browse this author →KAKEN DB
Uji, Toshiki Browse this author →KAKEN DB
Keywords: Pyropia
Red algae
Reproduction
1-aminocyclopropane-1-carboxylic acid
Plant growth regulator
Issue Date: Oct-2019
Publisher: Springer
Journal Title: Journal of Applied Phycology
Volume: 31
Issue: 5
Start Page: 3317
End Page: 3329
Publisher DOI: 10.1007/s10811-019-01808-7
Abstract: The marine red algal genus Pyropia, class Bangiophyceae, includes dioecious and monoecious species; however, the molecular mechanisms underlying control of their sexual reproduction are still poorly understood. In the present study, we demonstrated that application of the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promoted the formation of spermatangia and parthenosporangia in male and female gametophytes, respectively, of the dioecious species Pyropia pseudolinearis. In addition, we determined expression profiles of ACC-responsive genes in the gametophytes during sexual reproduction using RNA-Seq and quantitative real-time PCR (qRT-PCR). Genes involved in the regulation of cell division and cell wall organization, such as high-mobility group (PpHMG) and glycosyltransferase family (PpGT14), were found to be upregulated in male and female gametophytes treated with ACC. In addition, the relatively rapid ACC-response of the vesicular-trafficking-related genes, flotillin (PpFLOT), charged multivesicular body protein 5 (PpCHMP5), and peptidase family S8 (PpS8) was shown to occur during male and female sexual reproduction. Expression levels of these six genes in the monoecious species P. yezoensis, which are homologs to ACC-responsive genes in P. pseudolinearis, also increased in gametophytes treated with ACC. These findings could provide new insights into the ACC-regulation of the sexual life cycle in Pyropia species.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/79387
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宇治 利樹

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