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Mass spectrometry imaging reveals differential localization of natural sunscreens in the mantle of the giant clam Tridacna crocea

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Title: Mass spectrometry imaging reveals differential localization of natural sunscreens in the mantle of the giant clam Tridacna crocea
Authors: Goto-Inoue, Naoko Browse this author
Sato, Tomohiko Browse this author
Morisasa, Mizuki Browse this author
Yamashita, Hiroshi Browse this author
Maruyama, Tadashi Browse this author
Ikeda, Hiroki Browse this author
Sakai, Ryuichi Browse this author →KAKEN DB
Issue Date: 20-Jan-2020
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 10
Start Page: 656
Publisher DOI: 10.1038/s41598-019-57296-9
Abstract: Giant clams have evolved to maximize sunlight utilization by their photosymbiotic partners, while affording them protection from harmful ultraviolet (UV) light. The presence of UV absorbing substances in the mantle is thought to be critical for light protection; however, the exact localization of such compounds remains unknown. Here, we applied a combination of UV liquid chromatography (LC), LC-mass spectrometry (MS), MS imaging, and UV micrography to localize UV absorbing substances in the giant clam Tridacna crocea. LC-MS analysis revealed that the animal contained three classes of mycosporines: progenitor, primary, and secondary mycosporines. MS imaging revealed that primary and secondary mycosporines were localized in the outermost layer of the mantle; whereas progenitor mycosporines were distributed throughout the mantle tissue. These findings were consistent with the results of UV micrography, which revealed that the surface layer of the mantle absorbed UV light at 320 +/- 10nm. This is the first report indicating that progenitor and primary mycosporines are metabolized to secondary mycosporines by the giant clam and that they are differentially localized in the surface layer of the mantle to protect the animal from UV light.
Rights: https://creativecommons.org/licenses/by/4.0
Type: article
URI: http://hdl.handle.net/2115/79432
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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