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Hokkaido University Collection of Scholarly and Academic Papers >
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Mass spectrometry imaging reveals differential localization of natural sunscreens in the mantle of the giant clam Tridacna crocea
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| Title: | Mass spectrometry imaging reveals differential localization of natural sunscreens in the mantle of the giant clam Tridacna crocea |
| Authors: | Goto-Inoue, Naoko Browse this author | | Sato, Tomohiko Browse this author | | Morisasa, Mizuki Browse this author | | Yamashita, Hiroshi Browse this author | | Maruyama, Tadashi Browse this author | | Ikeda, Hiroki Browse this author | | Sakai, Ryuichi Browse this author →KAKEN DB |
| Issue Date: | 20-Jan-2020 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 10 |
| Start Page: | 656 |
| Publisher DOI: | 10.1038/s41598-019-57296-9 |
| Abstract: | Giant clams have evolved to maximize sunlight utilization by their photosymbiotic partners, while affording them protection from harmful ultraviolet (UV) light. The presence of UV absorbing substances in the mantle is thought to be critical for light protection; however, the exact localization of such compounds remains unknown. Here, we applied a combination of UV liquid chromatography (LC), LC-mass spectrometry (MS), MS imaging, and UV micrography to localize UV absorbing substances in the giant clam Tridacna crocea. LC-MS analysis revealed that the animal contained three classes of mycosporines: progenitor, primary, and secondary mycosporines. MS imaging revealed that primary and secondary mycosporines were localized in the outermost layer of the mantle; whereas progenitor mycosporines were distributed throughout the mantle tissue. These findings were consistent with the results of UV micrography, which revealed that the surface layer of the mantle absorbed UV light at 320 +/- 10nm. This is the first report indicating that progenitor and primary mycosporines are metabolized to secondary mycosporines by the giant clam and that they are differentially localized in the surface layer of the mantle to protect the animal from UV light. |
| Rights: | https://creativecommons.org/licenses/by/4.0 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79432 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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