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Establishment of an experimental model for MHC homo-to-hetero transplantation
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| Title: | Establishment of an experimental model for MHC homo-to-hetero transplantation |
| Authors: | Murata, Tomoki Browse this author | | Wada, Haruka Browse this author →KAKEN DB | | Otsuka, Ryo Browse this author | | Sasaki, Airi Browse this author | | Tsuji, Hyuma Browse this author | | Itoh, Mizuho Browse this author | | Eguchi, Nanami Browse this author | | Kawai, Tatsuo Browse this author | | Seino, Ken-ichiro Browse this author →KAKEN DB |
| Issue Date: | 11-Aug-2020 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 10 |
| Issue: | 1 |
| Start Page: | 13560 |
| Publisher DOI: | 10.1038/s41598-020-69784-4 |
| Abstract: | Preventing rejection is a major challenge in transplantation medicine, even when using pluripotent stem cell-derived grafts. In iPS cell (iPSC)-based transplantation, to reduce the risk of rejection, it is thought to be optimal that preparing the cells from donors whose human leukocyte antigen-haplotype are homozygous. Generally, this approach is referred to as major histocompatibility complex (MHC) homo-to-hetero transplantation, which is MHC-matched but minor antigen-mismatched. To investigate the immune response in the MHC homo-to-hetero transplantation, we established a murine experimental system in which MHC-matched but minor antigen-mismatched tissue (skin) grafts were transplanted into MHC-heterozygous recipients. Unexpectedly, only minor antigen-mismatched grafts were rejected at the same time points as rejection of fully allogeneic grafts. A vigorous anti-donor type T cell response was detected in vitro and conventional immunosuppressants targeting T cell activation had limited effects on controlling rejection. However, anti-donor antibodies were not detected only in the minor antigen-mismatched transplantation. This murine transplantation model can be used to further analyze immunological subjects for MHC homo-to-hetero iPSC-based transplantation. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79478 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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