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Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling

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Title: Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling
Authors: Yonamine, Yusuke Browse this author →KAKEN DB
Hiramatsu, Kotaro Browse this author
Ideguchi, Takuro Browse this author
Ito, Takuro Browse this author
Fujiwara, Tomomi Browse this author
Miura, Yoshiko Browse this author
Goda, Keisuke Browse this author
Hoshino, Yu Browse this author
Issue Date: 28-Apr-2020
Publisher: Royal Society of Chemistry
Journal Title: RSC advances
Volume: 10
Issue: 28
Start Page: 16679
End Page: 16686
Publisher DOI: 10.1039/d0ra02803g
Abstract: Cellular metabolites are valuable in a diverse range of applications. For example, the unicellular green algaHaematococcus lacustrisproduces as a secondary metabolite the carotenoid pigment astaxanthin (AXT), which is widely used in nutraceutical, cosmetic, and food industries due to its strong antioxidant activity. In order to enhance the productivity ofH. lacustris, spatial and temporal understanding of its metabolic dynamics is essential. Here we show spatiotemporal monitoring of AXT production inH. lacustriscells by resonance Raman microscopy combined with stable isotope labeling. Specifically, we incorporated carbon dioxide ((CO2)-C-13) labeled with a stable isotope (C-13) intoH. lacustriscells through carbon fixation and traced its conversion to(13)C-AXT using our resonance Raman microscope. We incubatedH. lacustriscells under various conditions by switching, pulsing, and replacing(13)CO(2)and(12)CO(2). By measurement of these cells we determined the fixation time of(13)C-carbon, visualized the intracellular localization of(13)C- and(12)C-AXTs, and revealed the dynamic consumption-production equilibrium of the accumulated AXT. This work is a valuable step in the development of effective screening criteria for high AXT-producingH. lacustriscells.
Type: article
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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