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Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats

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Title: Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats
Authors: Hasegawa, Tomoka Browse this author →KAKEN DB
Miyamoto-Takasaki, Yukina Browse this author
Abe, Miki Browse this author
Qiu, Zixuan Browse this author
Yamamoto, Tomomaya Browse this author
Yimin Browse this author
Yoshida, Taiji Browse this author
Yoshino, Hirona Browse this author
Hongo, Hiromi Browse this author →KAKEN DB
Yokoyama, Ayako Browse this author
Sasaki, Muneteru Browse this author
Kuroshima, Shinichiro Browse this author
Hara, Kuniko Browse this author
Kobayashi, Masatoshi Browse this author
Akiyama, Yasuhiro Browse this author
Maeda, Takeyasu Browse this author
Luiz de Freitas, Paulo Henrique Browse this author
Li, Minqi Browse this author
Amizuka, Norio Browse this author →KAKEN DB
Keywords: periodontal ligament
ascorbic acid
collagen fibril
reticular fibril
Issue Date: 9-Oct-2019
Publisher: Oxford University Press
Journal Title: Microscopy
Volume: 68
Issue: 5
Start Page: 349
End Page: 358
Publisher DOI: 10.1093/jmicro/dfz021
Abstract: In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)/ShiJcl-od/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS/ShiJcl-od/od male rats were administered ascorbic acid solution at a concentration of 200 mg/dL (control group, n = 6) or ascorbic acid solution at concentration of 0.3 mg/dL (insufficient group, n = 12). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200 mg/dL for additional 3 weeks (rescued group, n = 6), and then, their mandibles were histochemically examined. Consequently, the insufficient group specimens were seen to possess fewer collagen fibers, and silver impregnation revealed numerous fine, reticular fiber-like fibrils branching off from collagen in the periodontal ligaments. In control group, faint immunoreactivities for matrix metalloproteinase (MMP)2 and cathepsin H were seen in the periphery of blood vessels and throughout the ligament, respectively. In contrast, in the insufficient group, intense MMP2-immunoreactivity was observed to be associated with collagen fibrils in the periodontal ligaments, and cathepsin H-immunopositivity was seen in ligamentous cells. The rescued group showed abundant collagen fibers filling the periodontal ligament space. Under transmission electron microscopy, ligamentous fibroblasts incorporated collagen fibrils into tubular endosomes/lysosomes while simultaneously synthesizing collagen fibril bundles. Thus, ascorbic acid insufficiency affected the immunolocalization of cathepsin H and MMP2; however, ligamentous fibroblasts appear to possess the potential to synthesize collagen fibers when supplied with ascorbic acid.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in [Microscopy] following peer review. The version of record [Hasegawa, Tomoka; Miyamoto-Takasaki, Yukina; Abe, Miki; Qiu, Zixuan; Yamamoto, Tomomaya; Yimin; Yoshida, Taiji; Yoshino, Hirona; Hongo, Hiromi; Yokoyama, Ayako; Sasaki, Muneteru; Kuroshima, Shinichiro; Hara, Kuniko; Kobayashi, Masatoshi; Akiyama, Yasuhiro; Maeda, Takeyasu; Luiz de Freitas, Paulo Henrique; Li, Minqi; Amizuka, Norio; Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats. Microscopy, Volume 68, Issue 5, 9 October 2019, Pages 349–358] is available online at: [].
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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