Title: | Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification |
Authors: | Fukumoto, Tatsuya Browse this author |
Iwasaki, Sumio Browse this author |
Fujisawa, Shinichi Browse this author |
Hayasaka, Kasumi Browse this author |
Sato, Kaori Browse this author |
Oguri, Satoshi Browse this author |
Taki, Keisuke Browse this author |
Nakakubo, Sho Browse this author |
Kamada, Keisuke Browse this author →KAKEN DB |
Yamashita, Yu Browse this author |
Konno, Satoshi Browse this author →KAKEN DB |
Nishida, Mutsumi Browse this author →KAKEN DB |
Sugita, Junichi Browse this author →KAKEN DB |
Teshima, Takanori Browse this author →KAKEN DB |
Keywords: | COVID-19 |
SARS-CoV-2 |
PCR |
Saliva |
Issue Date: | Sep-2020 |
Publisher: | Elsevier |
Journal Title: | International journal of infectious diseases |
Volume: | 98 |
Start Page: | 16 |
End Page: | 17 |
Publisher DOI: | 10.1016/j.ijid.2020.06.074 |
Abstract: | Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time byeliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. |
Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/79584 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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