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Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification

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Title: Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification
Authors: Fukumoto, Tatsuya Browse this author
Iwasaki, Sumio Browse this author
Fujisawa, Shinichi Browse this author
Hayasaka, Kasumi Browse this author
Sato, Kaori Browse this author
Oguri, Satoshi Browse this author
Taki, Keisuke Browse this author
Nakakubo, Sho Browse this author
Kamada, Keisuke Browse this author →KAKEN DB
Yamashita, Yu Browse this author
Konno, Satoshi Browse this author →KAKEN DB
Nishida, Mutsumi Browse this author →KAKEN DB
Sugita, Junichi Browse this author →KAKEN DB
Teshima, Takanori Browse this author →KAKEN DB
Keywords: COVID-19
SARS-CoV-2
PCR
Saliva
Issue Date: Sep-2020
Publisher: Elsevier
Journal Title: International journal of infectious diseases
Volume: 98
Start Page: 16
End Page: 17
Publisher DOI: 10.1016/j.ijid.2020.06.074
Abstract: Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time byeliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/79584
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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