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Flexible NAD(+)Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A

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Title: Flexible NAD(+)Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A
Authors: Chen, Meirong Browse this author
Gai, Zuoqi Browse this author
Okada, Chiaki Browse this author
Ye, Yuxin Browse this author
Yu, Jian Browse this author
Yao, Min Browse this author →KAKEN DB
Keywords: IF5A
translation factor
hypusine modification
deoxyhypusine synthase
Issue Date: Aug-2020
Publisher: MDPI
Journal Title: International Journal of Molecular Sciences
Volume: 21
Issue: 15
Start Page: 5509
Publisher DOI: 10.3390/ijms21155509
Abstract: The eukaryotic and archaeal translation factor IF5A requires a post-translational hypusine modification, which is catalyzed by deoxyhypusine synthase (DHS) at a single lysine residue of IF5A with NAD(+)and spermidine as cofactors, followed by hydroxylation to form hypusine. While human DHS catalyzed reactions have been well characterized, the mechanism of the hypusination of archaeal IF5A by DHS is not clear. Here we report a DHS structure fromPyrococcus horikoshii OT3(PhoDHS) at 2.2 angstrom resolution. The structure reveals two states in a single functional unit (tetramer): two NAD(+)-bound monomers with the NAD(+)and spermidine binding sites observed in multi-conformations (closed and open), and two NAD(+)-free monomers. The dynamic loop region V288-P299, in the vicinity of the active site, adopts different positions in the closed and open conformations and is disordered when NAD(+)is absent. Combined with NAD(+)binding analysis, it is clear thatPhoDHS can exist in three states: apo,PhoDHS-2 equiv NAD(+), andPhoDHS-4 equiv NAD(+), which are affected by the NAD(+)concentration. Our results demonstrate the dynamic structure ofPhoDHS at the NAD(+)and spermidine binding site, with conformational changes that may be the response to the local NAD(+)concentration, and thus fine-tune the regulation of the translation process via the hypusine modification of IF5A.
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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