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Hibernating bear serum hinders osteoclastogenesis in-vitro

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Title: Hibernating bear serum hinders osteoclastogenesis in-vitro
Authors: Nasoori, Alireza Browse this author
Okamatsu-Ogura, Yuko Browse this author
Shimozuru, Michito Browse this author →KAKEN DB
Sashika, Mariko Browse this author →KAKEN DB
Tsubota, Toshio Browse this author →KAKEN DB
Issue Date: 27-Aug-2020
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 15
Issue: 8
Start Page: e0238132
Publisher DOI: 10.1371/journal.pone.0238132
Abstract: Bears do not suffer from osteoporosis during hibernation, which is associated with long-term inactivity, lack of food intake, and cold exposure. However, the mechanisms involved in bone loss prevention have scarcely been elucidated in bears. We investigated the effect of serum from hibernating Japanese black bears (Ursus thibetanus japonicus) on differentiation of peripheral blood mononuclear cells (PBMCs) to osteoclasts (OCs). PBMCs collected from 3 bears were separately cultured with 10% serum of 4 active and 4 hibernating bears (each individual serum type was assessed separately by a bear PBMCs), and differentiation were induced by treatment with macrophage colony stimulating factor (M-CSF) and receptor activator of NF-kB ligand (RANKL). PBMCs that were cultured with the active bear serum containing medium (ABSM) differentiated to multi-nucleated OCs, and were positive for TRAP stain. However, cells supplemented with hibernating bear serum containing medium (HBSM) failed to form OCs, and showed significantly lower TRAP stain (p< 0.001). On the other hand, HBSM induced proliferation of adipose derived mesenchymal stem cells (ADSCs) similarly to ABSM (p> 0.05), indicating no difference on cell growth. It was revealed that osteoclastogenesis of PBMCs is hindered by HBSM, implying an underlying mechanism for the suppressed bone resorption during hibernation in bears. In addition, this study for the first time showed the formation of bears' OCsin-vitro.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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