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Formation of a PSI-PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens

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Title: Formation of a PSI-PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens
Authors: Furukawa, Ryo Browse this author
Aso, Michiki Browse this author
Fujita, Tomomichi Browse this author
Akimoto, Seiji Browse this author
Tanaka, Ryouichi Browse this author →KAKEN DB
Tanaka, Ayumi Browse this author
Yokono, Makio Browse this author
Takabayashi, Atsushi Browse this author →KAKEN DB
Keywords: Physcomitrella
PSI-PSII megacomplex
Issue Date: 20-Sep-2019
Publisher: Springer
Journal Title: Journal of plant research
Volume: 132
Issue: 6
Start Page: 867
End Page: 880
Publisher DOI: 10.1007/s10265-019-01138-2
Abstract: Mosses are one of the earliest land plants that diverged from fresh-water green algae. They are considered to have acquired a higher capacity for thermal energy dissipation to cope with dynamically changing solar irradiance by utilizing both the "algal-type" light-harvesting complex stress-related (LHCSR)-dependent and the "plant-type" PsbS-dependent mechanisms. It is hypothesized that the formation of photosystem (PS) I and II megacomplex is another mechanism to protect photosynthetic machinery from strong irradiance. Herein, we describe the analysis of the PSI-PSII megacomplex from the model moss, Physcomitrella patens, which was resolved using large-pore clear-native polyacrylamide gel electrophoresis (lpCN-PAGE). The similarity in the migration distance of the Physcomitrella PSI-PSII megacomplex to the Arabidopsis megacomplex shown during lpCN-PAGE suggested that the Physcomitrella PSI-PSII and Arabidopsis megacomplexes have similar structures. Time-resolved chlorophyll fluorescence measurements show that excitation energy was rapidly and efficiently transferred from PSII to PSI, providing evidence of an ordered association of the two photosystems. We also found that LHCSR and PsbS co-migrated with the Physcomitrella PSI-PSII megacomplex. The megacomplex showed pH-dependent chlorophyll fluorescence quenching, which may have been induced by LHCSR and/or PsbS proteins with the collaboration of zeaxanthin. We discuss the mechanism that regulates the energy distribution balance between two photosystems in Physcomitrella.
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Type: article (author version)
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高林 厚史

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