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Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes

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Title: Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes
Authors: Thompson, Lesa A. Browse this author
Ikenaka, Yoshinori Browse this author →KAKEN DB
Darwish, Wageh Sobhy Browse this author
Nakayama, Shouta M. M. Browse this author →KAKEN DB
Mizukawa, Hazuki Browse this author →KAKEN DB
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: p,p '-DDT
MCF-7 cells
Transcriptomics
Biomarkers
Issue Date: 29-Aug-2019
Publisher: Elsevier
Journal Title: Environmental toxicology and pharmacology
Volume: 72
Start Page: UNSP 103249
Publisher DOI: 10.1016/j.etap.2019.103249
Abstract: The organochlorine pesticide dichloro-diphenyl-trichloroethane (DDT) is persistent in the environment and leads to adverse human health effects. High levels in breast milk pose a threat to both breast tissue and nursing infants. The objectives of this study were to investigate DDT-induced transcriptomic alterations in enzymes and transporters involved in xenobiotic metabolism, immune responses, oxidative stress markers, and cell growth in a human breast cancer cell line. MCF-7 cells were exposed to both environmentally-relevant and previously-tested concentrations of p,p'-DDT in a short-term experiment. Significant up-regulation of metabolizing enzymes and transporters (ACHE, GSTO1, NQO1 and ABCC2) and oxidative stress markers (CXCL8, HMOX-1, NFE2L2 and TNF) was clearly observed. Conversely, UGT1A6, AHR and cell growth genes (FGF2 and VEGFA) were severely down-regulated. Identification of these genes helps to identify mechanisms of p,p'-DDT action within cells and may be considered as useful biomarkers for exposure to DDT contamination.
Rights: © 2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/79687
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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