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Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes
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| Title: | Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes |
| Authors: | Thompson, Lesa A. Browse this author | | Ikenaka, Yoshinori Browse this author →KAKEN DB | | Darwish, Wageh Sobhy Browse this author | | Nakayama, Shouta M. M. Browse this author →KAKEN DB | | Mizukawa, Hazuki Browse this author →KAKEN DB | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | p,p '-DDT | | MCF-7 cells | | Transcriptomics | | Biomarkers |
| Issue Date: | 29-Aug-2019 |
| Publisher: | Elsevier |
| Journal Title: | Environmental toxicology and pharmacology |
| Volume: | 72 |
| Start Page: | UNSP 103249 |
| Publisher DOI: | 10.1016/j.etap.2019.103249 |
| Abstract: | The organochlorine pesticide dichloro-diphenyl-trichloroethane (DDT) is persistent in the environment and leads to adverse human health effects. High levels in breast milk pose a threat to both breast tissue and nursing infants. The objectives of this study were to investigate DDT-induced transcriptomic alterations in enzymes and transporters involved in xenobiotic metabolism, immune responses, oxidative stress markers, and cell growth in a human breast cancer cell line. MCF-7 cells were exposed to both environmentally-relevant and previously-tested concentrations of p,p'-DDT in a short-term experiment. Significant up-regulation of metabolizing enzymes and transporters (ACHE, GSTO1, NQO1 and ABCC2) and oxidative stress markers (CXCL8, HMOX-1, NFE2L2 and TNF) was clearly observed. Conversely, UGT1A6, AHR and cell growth genes (FGF2 and VEGFA) were severely down-regulated. Identification of these genes helps to identify mechanisms of p,p'-DDT action within cells and may be considered as useful biomarkers for exposure to DDT contamination. |
| Rights: | © 2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79687 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石塚 真由美
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