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Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis

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Title: Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis
Authors: Aiyama, Takeshi Browse this author
Orimo, Tatsuya Browse this author →KAKEN DB
Yokoo, Hideki Browse this author →KAKEN DB
Ohata, Takanori Browse this author
Hatanaka, Kanako C. Browse this author
Hatanaka, Yutaka Browse this author
Fukai, Moto Browse this author →KAKEN DB
Kamiyama, Toshiya Browse this author →KAKEN DB
Taketomi, Akinobu Browse this author →KAKEN DB
Issue Date: 21-Sep-2020
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 15
Issue: 9
Start Page: e0239462
Publisher DOI: 10.1371/journal.pone.0239462
Abstract: This study was performed to determine the clinical significance of adenomatous polyposis coli (APC)-binding protein end-binding 1 (EB1) in hepatocellular carcinoma (HCC) and to characterize its biochemical role in comparison with previous reports. We performed immunohistochemical staining to detect EB1 expression in tissues from 235 patients with HCC and investigated its correlations with clinicopathological features and prognosis. We also investigated the roles of EB1 in cell proliferation, migration, and tumorigenesisin vitroandin vivoby siRNA- and CRISPR/Cas9-mediated modulation of EB1 expression in human HCC cell lines. The results showed that EB1 expression was significantly correlated with several important factors associated with tumor malignancy, including histological differentiation, portal vein invasion status, and intrahepatic metastasis. Patients with high EB1 expression in HCC tissue had poorer overall survival and higher recurrence rates than patients with low EB1 expression. EB1 knockdown and knockout in HCC cells reduced cell proliferation, migration, and invasionin vitroand inhibited tumor growthin vivo. Further, genes encoding Dlk1, HAMP, and SLCO1B3 that were differentially expressed in association with EB1 were identified using RNA microarray analysis. In conclusion, elevated expression of EB1 promotes tumor growth and metastasis of HCC. EB1 may serve as a new biomarker for HCC, and genes coexpressed with EB1 may represent potential targets for therapy.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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