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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis
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| Title: | Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis |
| Authors: | Aiyama, Takeshi Browse this author | | Orimo, Tatsuya Browse this author →KAKEN DB | | Yokoo, Hideki Browse this author →KAKEN DB | | Ohata, Takanori Browse this author | | Hatanaka, Kanako C. Browse this author | | Hatanaka, Yutaka Browse this author | | Fukai, Moto Browse this author →KAKEN DB | | Kamiyama, Toshiya Browse this author →KAKEN DB | | Taketomi, Akinobu Browse this author →KAKEN DB |
| Issue Date: | 21-Sep-2020 |
| Publisher: | PLOS |
| Journal Title: | PLoS ONE |
| Volume: | 15 |
| Issue: | 9 |
| Start Page: | e0239462 |
| Publisher DOI: | 10.1371/journal.pone.0239462 |
| Abstract: | This study was performed to determine the clinical significance of adenomatous polyposis coli (APC)-binding protein end-binding 1 (EB1) in hepatocellular carcinoma (HCC) and to characterize its biochemical role in comparison with previous reports. We performed immunohistochemical staining to detect EB1 expression in tissues from 235 patients with HCC and investigated its correlations with clinicopathological features and prognosis. We also investigated the roles of EB1 in cell proliferation, migration, and tumorigenesisin vitroandin vivoby siRNA- and CRISPR/Cas9-mediated modulation of EB1 expression in human HCC cell lines. The results showed that EB1 expression was significantly correlated with several important factors associated with tumor malignancy, including histological differentiation, portal vein invasion status, and intrahepatic metastasis. Patients with high EB1 expression in HCC tissue had poorer overall survival and higher recurrence rates than patients with low EB1 expression. EB1 knockdown and knockout in HCC cells reduced cell proliferation, migration, and invasionin vitroand inhibited tumor growthin vivo. Further, genes encoding Dlk1, HAMP, and SLCO1B3 that were differentially expressed in association with EB1 were identified using RNA microarray analysis. In conclusion, elevated expression of EB1 promotes tumor growth and metastasis of HCC. EB1 may serve as a new biomarker for HCC, and genes coexpressed with EB1 may represent potential targets for therapy. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79691 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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