HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >

Expression and Localization of Paneth Cells and Their alpha-Defensins in the Small Intestine of Adult Mouse

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:

Title: Expression and Localization of Paneth Cells and Their alpha-Defensins in the Small Intestine of Adult Mouse
Authors: Ohira, Shuya Browse this author
Shinozaki, Ryuga Browse this author
Nishida, Takuto Browse this author
Kikuchi, Mani Browse this author
Ayabe, Tokiyoshi Browse this author
Nakamura, Kiminori Browse this author
Yokoi, Yuki Browse this author
Fukaya, Rie Browse this author
Keywords: Paneth cell
Institute of Cancer Research mouse
innate immunity
germ-free mouse
Issue Date: Oct-2020
Publisher: Frontiers Media
Journal Title: Frontiers in Immunology
Volume: 11
Start Page: 570296
Publisher DOI: 10.3389/fimmu.2020.570296
PMID: 33154750
Abstract: Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting alpha-defensin. In Institute of Cancer Research (ICR) mice, alpha-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that alpha-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of alpha-defensin remains to be determined. In this study, we examined the expression level of messenger RNA (mRNA) encoding six Crp-isoforms and Crp immunoreactivities using singly isolated crypts together with bactericidal activities of Paneth cell secretions from isolated crypts of duodenum, jejunum, and ileum. Here we showed that levels of Crp mRNAs in the single crypt ranged from 5 x 10(3) to 1 x 10(6) copies per 5 ng RNA. For each Crp isoform, the expression level in ileum was 4 to 50 times higher than that in duodenum and jejunum. Furthermore, immunohistochemical analysis of isolated crypts revealed that the average number of Paneth cell per crypt in the small intestine increased from proximal to distal, three to seven-fold, respectively. Both Crp1 and 4 expressed greater in ileal Paneth cells than those in duodenum or jejunum. Bactericidal activities in secretions of ileal Paneth cell exposed to bacteria were significantly higher than those of duodenum or jejunum. In germ-free mice, Crp expression in each site of the small intestine was attenuated and bactericidal activities released by ileal Paneth cells were decreased compared to those in conventional mice. Taken together, Paneth cells and their alpha-defensin in adult mouse appeared to be regulated topographically in innate immunity to control intestinal integrity.
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 - Hokkaido University