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Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis

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Title: Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis
Authors: Izumi, Hikari Browse this author →KAKEN DB
Gen, Koichiro Browse this author →KAKEN DB
Lokman, P. Mark Browse this author
Hagihara, Seishi Browse this author →KAKEN DB
Horiuchi, Moemi Browse this author
Tanaka, Toshiomi Browse this author
Ijiri, Shigeho Browse this author →KAKEN DB
Adachi, Shinji Browse this author →KAKEN DB
Keywords: development
Japanese eel
maternal transcript
qPCR
Issue Date: Dec-2019
Publisher: John Wiley & Sons
Journal Title: Molecular Reproduction and Development
Publisher DOI: 10.1002/mrd.23273
Abstract: Our understanding of maternal control of development in vertebrates remains incomplete. In this study, we investigated levels of maternal transcripts in good and poor quality eggs from artificially matured Japanese eel, using RNA-Seq and quantitative polymerase chain reaction (qPCR), to identify candidate maternal transcripts related to development. De novo assembly or mapping of reads to the eel draft genome yielded 619,029 contigs and 85,906 transcripts, respectively; normalized read counts to these assemblies were calculated using reads (RPKM) or fragments (FPKM) per kilobase of transcript per million mapped reads. In silico screening identified 1,594 contigs and 150 transcripts with lower RPKM or FPKM in poor than in good quality eggs, 245 contigs, and 85 transcripts of which could be annotated by BLASTx, respectively. From selected contigs or transcripts, six genes (dnajb4, gnpat, card14, pdp1, fcgbp, ttn) had significantly lower messenger RNA levels in poor than in good quality eggs by qPCR. Multiple regression analysis showed that five genes (gnpat, b4galnt1, acsl6, rtkn, trim24) significantly correlated with hatchability. Taken together, 10 genes were identified as candidate maternal transcripts, regulating development in Japanese eel. Our results contribute to understanding the molecular basis for maternal control of development in vertebrates.
Rights: This is the peer reviewed version of the following article: Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis, which has been published in final form at https://doi.org/10.1002/mrd.23273. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/79855
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 井尻 成保

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