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Hokkaido University Collection of Scholarly and Academic Papers >
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High drug efflux pump capacity and low DNA damage response induce doxorubicin resistance in canine hemangiosarcoma cell lines
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| Title: | High drug efflux pump capacity and low DNA damage response induce doxorubicin resistance in canine hemangiosarcoma cell lines |
| Authors: | Morita, Atsuya Browse this author | | Aoshima, Keisuke Browse this author | | Gulay, Kevin Christian Montecillo Browse this author | | Onishi, Shinichi Browse this author | | Shibata, Yuki Browse this author | | Yasui, Hironobu Browse this author | | Kobayashi, Atsushi Browse this author | | Kimura, Takashi Browse this author |
| Keywords: | Apoptosis | | Cancer | | DNA damage response | | Doxorubicin | | Drug resistance | | Hemangiosarcoma |
| Issue Date: | Dec-2019 |
| Publisher: | Elsevier |
| Journal Title: | Research in veterinary science |
| Volume: | 127 |
| Start Page: | 1 |
| End Page: | 10 |
| Publisher DOI: | 10.1016/j.rvsc.2019.09.011 |
| Abstract: | Canine hemangiosarcoma (HSA) is an aggressive malignant endothelial tumor in dogs and characterized by poor prognosis because of its high invasiveness, high metastatic potential, and poor responsiveness to anti-cancer drugs. Although doxorubicin-based chemotherapy is regularly conducted after surgical treatment, its effects on survival rates are limited. Acquisition of drug resistance is one of the causes of this problem, but the underlying mechanisms remain unclear. In the present study, we aimed to identify the drug-resistance mechanism in canine HSA by establishing doxorubicin-resistant (DR) HSA cell lines. HSA cell lines were exposed to doxorubicin at gradually increasing concentrations. When the cells were able to grow in the presence of a 16-fold higher doxorubicin concentration compared with the initial culture, they were designated DR-HSA cell lines. Characterization of these DR-HSA cell lines revealed higher drug efflux pump capacity compared with the parental cell lines. Furthermore, the DR-HSA cell lines did not show activation of the DNA damage response despite carrying high DNA damage burdens, meaning that apoptosis was not strongly induced. In conclusion, canine HSA cell lines acquired doxorubicin resistance by increasing their drug efflux pump capacity and decreasing the DNA damage response. This study provides useful findings to promote further research on the drug-resistance mechanisms in canine HSA. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79880 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 青島 圭佑
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