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The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

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Title: The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti
Authors: Runtuwene, Lucky R. Browse this author
Kawashima, Shuichi Browse this author
Pijoh, Victor D. Browse this author
Tuda, Josef S. B. Browse this author
Hayashida, Kyoko Browse this author
Yamagishi, Junya Browse this author
Sugimoto, Chihiro Browse this author
Nishiyama, Shoko Browse this author
Sasaki, Michihito Browse this author
Orba, Yasuko Browse this author
Sawa, Hirofumi Browse this author
Takasaki, Tomohiko Browse this author
James, Anthony A. Browse this author
Kobayashi, Takashi Browse this author
Eshita, Yuki Browse this author →KAKEN DB
Keywords: lethal(2)-essential-for-life
dengue
Aedes aegypti
Issue Date: Oct-2020
Publisher: MDPI
Journal Title: International Journal of Molecular Sciences
Volume: 21
Issue: 20
Start Page: 7520
Publisher DOI: 10.3390/ijms21207520
Abstract: Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2 alpha) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2 alpha remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/79916
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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