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Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia
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| Title: | Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia |
| Authors: | Wastika, Christida E. Browse this author | | Harima, Hayato Browse this author | | Sasaki, Michihito Browse this author | | Hang'ombe, Bernard M. Browse this author | | Eshita, Yuki Browse this author | | Qiu, Yongjin Browse this author | | Hall, William W. Browse this author | | Wolfinger, Michael T. Browse this author | | Sawa, Hirofumi Browse this author | | Orba, Yasuko Browse this author →KAKEN DB |
| Keywords: | Barkedji virus | | Barkedji-like virus | | dISFVs | | 5 '-UTR | | 3 '-UTR | | RNA secondary structure |
| Issue Date: | Sep-2020 |
| Publisher: | MDPI |
| Journal Title: | Viruses-Basel |
| Volume: | 12 |
| Issue: | 9 |
| Start Page: | 1017 |
| Publisher DOI: | 10.3390/v12091017 |
| Abstract: | To monitor the arthropod-borne virus transmission in mosquitoes, we have attempted both to detect and isolate viruses from 3304 wild-caught female mosquitoes in the Livingstone (Southern Province) and Mongu (Western Province) regions in Zambia in 2017. A pan-flavivirus RT-PCR assay was performed to identify flavivirus genomes in total RNA extracted from mosquito lysates, followed by virus isolation and full genome sequence analysis using next-generation sequencing and rapid amplification of cDNA ends. We isolated a newly identified Barkedji virus (BJV Zambia) (10,899 nt) and a novel flavivirus, tentatively termed Barkedji-like virus (BJLV) (10,885 nt) fromCulexspp. mosquitoes which shared 96% and 75% nucleotide identity with BJV which has been isolated in Israel, respectively. These viruses could replicate in C6/36 cells but not in mammalian and avian cell lines. In parallel, a comparative genomics screening was conducted to study evolutionary traits of the 5 '- and 3 '-untranslated regions (UTRs) of isolated viruses. Bioinformatic analyses of the secondary structures in the UTRs of both viruses revealed that the 5 '-UTRs exhibit canonical stem-loop structures, while the 3 '-UTRs contain structural homologs to exoribonuclease-resistant RNAs (xrRNAs), SL-III, dumbbell, and terminal stem-loop (3 ' SL) structures. The function of predicted xrRNA structures to stop RNA degradation by Xrn1 exoribonuclease was further proved by the in vitro Xrn1 resistance assay. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79920 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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