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Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst

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Title: Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst
Authors: Kohri, Nanami Browse this author
Akizawa, Hiroki Browse this author
Iisaka, Sakie Browse this author
Bai, Hanako Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Masashi Browse this author
Komatsu, Masaya Browse this author
Kawai, Masahito Browse this author
Nagano, Masashi Browse this author
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: blastocyst
gene expression
Hippo pathway
inner cell mass
protein-protein interaction
yes-associated protein
Issue Date: 13-Dec-2019
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 294
Issue: 50
Start Page: 19209
End Page: 19223
Publisher DOI: 10.1074/jbc.RA119.010746
Abstract: Which comes first: tissue structure or cell differentiation? While different cell types establish distinct structures delineating the in- and outside of an embryo, they progressively become specified by the blastocyst stage, when two types of cell lineages are formed: inner cell mass (ICM) and trophectoderm (TE). This inside-outside aspect can be experimentally converted by the isolation of the ICM from a blastocyst, leading to a posteriori externalization of the blastomeres composing the outermost layer of the ICM. Here, we investigated the totipotency of isolated mouse and bovine ICMs to determine whether they are competent for TE regeneration. Surprisingly, a calf was generated from the bovine isolated ICM with re-formed blastocoel (re-iICM), but no mouse re-iICMs developed to term. To further explore the cause of difference in developmental competency between the mouse and bovine re-iICMs, we investigated the SOX17 protein expression that is a representative molecular marker of primitive endoderm. The localization pattern of SOX17 was totally different between mouse and bovine embryos. Particularly, the ectopic SOX17 localization in the TE might be associated with lethality of mouse re-iICMs. Meanwhile, transcriptome sequencing revealed that some of the bovine re-iICMs showed transcriptional patterns of TE-specific genes similar to those of whole blastocysts. Our findings suggest that TE regeneration competency is maintained longer in bovine ICMs than in mouse ICMs and provide evidence that the ICM/TE cell fate decision is influenced by structural determinants, including positional information of each blastomere in mammalian embryos.
Rights: This research was originally published in the Journal of Biological Chemistry. Kohri Nanami, Akizawa Hiroki, Iisaka Sakie, Bai Hanako, Yanagawa Yojiro, Takahashi Masashi, Komatsu Masaya, Kawai Masahito, Nagano Masashi and Kawahara Manabu. Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst. J. Biol. Chem. 2019; Vol294:19209-19223. © the American Society for Biochemistry and Molecular Biology.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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