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Traditional Japanese Herbal Medicine Hochu-Ekki-to Promotes Pneumococcal Colonization Clearance via Macrophage Activation and Interleukin 17A Production in Mice
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| Title: | Traditional Japanese Herbal Medicine Hochu-Ekki-to Promotes Pneumococcal Colonization Clearance via Macrophage Activation and Interleukin 17A Production in Mice |
| Authors: | Nakakubo, Sho Browse this author | | Kimura, Soichiro Browse this author | | Mimura, Kazuyuki Browse this author | | Kajiwara, Chiaki Browse this author | | Ishii, Yoshikazu Browse this author | | Konno, Satoshi Browse this author →KAKEN DB | | Tateda, Kazuhiro Browse this author |
| Keywords: | traditional herbal medicine | | Hochu-ekki-to (TJ-41) | | pneumococcal colonization | | macrophage | | interleukin 17 | | innate immunity |
| Issue Date: | 22-Oct-2020 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in Cellular and Infection Microbiology |
| Volume: | 10 |
| Start Page: | 569158 |
| Publisher DOI: | 10.3389/fcimb.2020.569158 |
| Abstract: | Streptococcus pneumoniae may colonize the nasopharynx, and as pneumococcal colonization causes invasive diseases and the subsequent transmission, reducing bacterial burden in the nasal cavity is critical. Hochu-ekki-to (TJ-41) is a traditional Japanese herbal medicine that exerts immunomodulatory effects in host cells. In this study, we investigated the potency of TJ-41 in modulating pneumococcal colonization clearance by activating host immunity. Mice, intranasally inoculated with pneumococci, were treated orally with TJ-41. During colonization, TJ-41 treatment significantly reduced pneumococcal burden and increased macrophage population in the nasopharynx. Furthermore, interleukin 17A production was significantly enhanced after TJ-41 treatment. In vitro experiment using nasal-derived cells revealed that pneumococcal antigen exposure upregulated the transcription of interleukin 17A in the TJ-41-treated group compared with that in the control group. Macrophages activated by killed bacteria were significantly increased in the presence of TJ-41 in an interleukin 17A-dependent manner. Moreover, TJ-41 enhanced phagocytosis, inhibited bacterial growth, and improved the antigen-presenting capacity of macrophages. Our results demonstrate that TJ-41 accelerates the clearance of pneumococcal nasopharyngeal colonization via macrophage activation. Subsequent production of interleukin 17A provides an additional benefit to effector cells. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79999 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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