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Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

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Title: Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia
Authors: Harima, Hayato Browse this author
Sasaki, Michihito Browse this author →KAKEN DB
Kajihara, Masahiro Browse this author →KAKEN DB
Gonzalez, Gabriel Browse this author
Simulundu, Edgar Browse this author
Bwalya, Eugene C. Browse this author
Qiu, Yongjin Browse this author →KAKEN DB
Okuya, Kosuke Browse this author
Isono, Mao Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Hang'ombe, Bernard M. Browse this author
Mweene, Aaron S. Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: mammalian orthoreovirus
prevalence
complete genome
reassortment
pig
Zambia
Issue Date: 24-Jul-2020
Publisher: Society for General Microbiology
Journal Title: Journal of general virology
Volume: 101
Issue: 10
Start Page: 1027
End Page: 1036
Publisher DOI: 10.1099/jgv.0.001476
Abstract: Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7% (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the 53 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs. respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.
Rights: © Hayato Harima, Michihito Sasaki, Masahiro Kajihara, Gabriel Gonzalez, Edgar Simulundu, Eugene C. Bwalya, Yongjin Qiu​, Kosuke Okuya, Mao Isono, Yasuko Orba, Ayato Takada​, Bernard M. Hang’ombe​, Aaron S. Mweene​, Hirofumi Sawa, 2020. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, volume101, issue10, 2020, https://doi.org/10.1099/jgv.0.001476.
https://creativecommons.org/licenses/by/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/80078
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤 洋文

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