| Title: | Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer : A Novel Therapeutic Target |
| Authors: | Kushibiki, Toshihiro Browse this author |
| Nakamura, Toru Browse this author →KAKEN DB |
| Tsuda, Masumi Browse this author →KAKEN DB |
| Tsuchikawa, Takahiro Browse this author →KAKEN DB |
| Hontani, Koji Browse this author |
| Inoko, Kazuho Browse this author |
| Takahashi, Mizuna Browse this author |
| Asano, Toshimichi Browse this author →KAKEN DB |
| Okamura, Keisuke Browse this author →KAKEN DB |
| Murakami, Soichi Browse this author →KAKEN DB |
| Kurashima, Yo Browse this author →KAKEN DB |
| Ebihara, Yuma Browse this author →KAKEN DB |
| Noji, Takehiro Browse this author →KAKEN DB |
| Nakanishi, Yoshitsugu Browse this author →KAKEN DB |
| Tanaka, Kimitaka Browse this author →KAKEN DB |
| Maishi, Nako Browse this author →KAKEN DB |
| Sasaki, Katsunori Browse this author →KAKEN DB |
| Park, Woong-Ryeon Browse this author |
| Shichinohe, Toshiaki Browse this author →KAKEN DB |
| Hida, Kyoko Browse this author →KAKEN DB |
| Tanaka, Shinya Browse this author →KAKEN DB |
| Hirano, Satoshi Browse this author →KAKEN DB |
| Issue Date: | Jan-2020 |
| Publisher: | American Association for Cancer Research (AACR) |
| Journal Title: | Molecular cancer therapeutics |
| Volume: | 19 |
| Issue: | 1 |
| Start Page: | 187 |
| End Page: | 198 |
| Publisher DOI: | 10.1158/1535-7163.MCT-19-0491 |
| Abstract: | Over the past 30 years, the therapeutic outcome for pancreatic ductal adenocarcinoma (PDAC) has remained stagnant due to the lack of effective treatments. We performed a genome-wide analysis to identify novel therapeutic targets for PDAC. Our analysis showed that Homo sapiens chromosome 16 open reading frame 74 (C16orf74) was upregulated in most patients with PDAC and associated with poor prognosis. Previously, we demonstrated that C16orf74 interacts with the catalytic subunit alpha of protein phosphatase 3 and plays an important role in PDAC invasion. However, the pathophysiologic function of C16orf74 is still unclear. In this study, through the analysis of C16orf74 interaction, we demonstrate a new strategy to inhibit the growth and invasion of PDAC. C16orf74 exists in the homodimer form under the cell membrane and binds integrin alpha V beta 3 and is also involved in invasion by activating Rho family (Rac1) and MMP2. Considering that this dimeric form was found to be involved in the function of C16orf74, we designed an 11R-DB (dimer block) cell-permeable dominant-negative peptide that inhibits the dimer form of C16orf74. 11R-DB suppressed invasion and proliferation of PDAC cell lines by inhibiting phosphorylation of Akt and mTOR and also by inactivation of MMP2. 11R-DB also showed antitumor effects in an orthotopic xenograft model and peritoneal metastasis model. Thus, this study demonstrates that dimerized C16orf74, present in the cell membrane, is involved in pancreatic cancer invasion and proliferation. In addition, the C16orf74 dimer block cell-permeable peptide (11-RDB) has a potent therapeutic effect on PDAC in vitro and in vivo. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80090 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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