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Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer : A Novel Therapeutic Target

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Title: Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer : A Novel Therapeutic Target
Authors: Kushibiki, Toshihiro Browse this author
Nakamura, Toru Browse this author →KAKEN DB
Tsuda, Masumi Browse this author →KAKEN DB
Tsuchikawa, Takahiro Browse this author →KAKEN DB
Hontani, Koji Browse this author
Inoko, Kazuho Browse this author
Takahashi, Mizuna Browse this author
Asano, Toshimichi Browse this author →KAKEN DB
Okamura, Keisuke Browse this author →KAKEN DB
Murakami, Soichi Browse this author →KAKEN DB
Kurashima, Yo Browse this author →KAKEN DB
Ebihara, Yuma Browse this author →KAKEN DB
Noji, Takehiro Browse this author →KAKEN DB
Nakanishi, Yoshitsugu Browse this author →KAKEN DB
Tanaka, Kimitaka Browse this author →KAKEN DB
Maishi, Nako Browse this author →KAKEN DB
Sasaki, Katsunori Browse this author →KAKEN DB
Park, Woong-Ryeon Browse this author
Shichinohe, Toshiaki Browse this author →KAKEN DB
Hida, Kyoko Browse this author →KAKEN DB
Tanaka, Shinya Browse this author →KAKEN DB
Hirano, Satoshi Browse this author →KAKEN DB
Issue Date: Jan-2020
Publisher: American Association for Cancer Research (AACR)
Journal Title: Molecular cancer therapeutics
Volume: 19
Issue: 1
Start Page: 187
End Page: 198
Publisher DOI: 10.1158/1535-7163.MCT-19-0491
Abstract: Over the past 30 years, the therapeutic outcome for pancreatic ductal adenocarcinoma (PDAC) has remained stagnant due to the lack of effective treatments. We performed a genome-wide analysis to identify novel therapeutic targets for PDAC. Our analysis showed that Homo sapiens chromosome 16 open reading frame 74 (C16orf74) was upregulated in most patients with PDAC and associated with poor prognosis. Previously, we demonstrated that C16orf74 interacts with the catalytic subunit alpha of protein phosphatase 3 and plays an important role in PDAC invasion. However, the pathophysiologic function of C16orf74 is still unclear. In this study, through the analysis of C16orf74 interaction, we demonstrate a new strategy to inhibit the growth and invasion of PDAC. C16orf74 exists in the homodimer form under the cell membrane and binds integrin alpha V beta 3 and is also involved in invasion by activating Rho family (Rac1) and MMP2. Considering that this dimeric form was found to be involved in the function of C16orf74, we designed an 11R-DB (dimer block) cell-permeable dominant-negative peptide that inhibits the dimer form of C16orf74. 11R-DB suppressed invasion and proliferation of PDAC cell lines by inhibiting phosphorylation of Akt and mTOR and also by inactivation of MMP2. 11R-DB also showed antitumor effects in an orthotopic xenograft model and peritoneal metastasis model. Thus, this study demonstrates that dimerized C16orf74, present in the cell membrane, is involved in pancreatic cancer invasion and proliferation. In addition, the C16orf74 dimer block cell-permeable peptide (11-RDB) has a potent therapeutic effect on PDAC in vitro and in vivo.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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