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Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate

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Title: Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate
Authors: Kato, Souichiro Browse this author →KAKEN DB
Terashima, Mia Browse this author
Yama, Ayano Browse this author
Sato, Megumi Browse this author
Kitagawa, Wataru Browse this author
Kawasaki, Kosei Browse this author
Kamagata, Yoichi Browse this author
Keywords: culturability
anaerobic bacteria
wastewater treatment plant
agar medium preparation
phosphate
Issue Date: 13-Jan-2021
Publisher: Japanese Society of Microbial Ecology, Department of Bioresource Science
Journal Title: Microbes and environments
Volume: 35
Issue: 1
Start Page: ME19060
Publisher DOI: 10.1264/jsme2.ME19060
Abstract: We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the "PS protocol"), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numbers and the frequency of phylogenctically novel isolates under aerobic, nitrate reduction, and fermentation conditions. The PS protocol is useful for isolating both aerobic and anaerobic microorganisms.
Type: article
URI: http://hdl.handle.net/2115/80146
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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