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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate
| Title: | Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate |
| Authors: | Kato, Souichiro Browse this author →KAKEN DB | | Terashima, Mia Browse this author | | Yama, Ayano Browse this author | | Sato, Megumi Browse this author | | Kitagawa, Wataru Browse this author | | Kawasaki, Kosei Browse this author | | Kamagata, Yoichi Browse this author |
| Keywords: | culturability | | anaerobic bacteria | | wastewater treatment plant | | agar medium preparation | | phosphate |
| Issue Date: | 13-Jan-2021 |
| Publisher: | Japanese Society of Microbial Ecology, Department of Bioresource Science |
| Journal Title: | Microbes and environments |
| Volume: | 35 |
| Issue: | 1 |
| Start Page: | ME19060 |
| Publisher DOI: | 10.1264/jsme2.ME19060 |
| Abstract: | We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the "PS protocol"), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numbers and the frequency of phylogenctically novel isolates under aerobic, nitrate reduction, and fermentation conditions. The PS protocol is useful for isolating both aerobic and anaerobic microorganisms. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80146 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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