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Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species

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Title: Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species
Authors: Kuniyoshi, Daichi Browse this author
Masuda, Itaru Browse this author
Kanaoka, Yoshitaka Browse this author
Shimazaki-Kishi, Yuki Browse this author
Okamoto, Yoshihiro Browse this author
Yasui, Hideshi Browse this author
Yamamoto, Toshio Browse this author
Nagaki, Kiyotaka Browse this author
Hoshino, Yoichiro Browse this author
Koide, Yohei Browse this author
Takamure, Itsuro Browse this author
Kishima, Yuji Browse this author →KAKEN DB
Keywords: anther culture
division restitution
hybrid sterility
interspecific hybrid
meiosis
rice
tetraploid
diploid gamete
Issue Date: 5-Nov-2020
Publisher: Frontiers Media
Journal Title: Frontiers in plant science
Volume: 11
Start Page: 579305
Publisher DOI: 10.3389/fpls.2020.579305
Abstract: In F-1 hybrids of Oryza sativa (Asian rice) and Oryza glaberrima (African rice), heterozygosity leads to a complete gamete abortion because of allelic conflict at each of the 13 hybrid sterility (HS) loci. We systematically produced 19 plants from the F-1 hybrids of both the rice species by the anther culture (AC) method. Five of the 19 interspecific hybrid plants were partially fertile and able to produce seeds. Unlike ordinal doubled haploid plants resulting from AC, these regenerated plants showed various ploidy levels (diploid to pentaploid) and different zygosities (completely homozygous, completely heterozygous, and a combination). These properties were attributable to meiotic anomalies in the interspecific hybrid F-1 plants. Examination of the genetic structures of the regenerated plants suggested meiotic non-reduction took place in the interspecific hybrid F-1 plants. The centromeric regions in the regenerated plants revealed that the abnormal first and/or second divisions of meiosis, namely the first division restitution (FDR) and/or second division restitution (SDR), had occurred in the interspecific hybrid. Immunohistochemical observations also verified these phenomena. FDR and SDR occurrences at meiosis might strongly lead to the formation of diploid microspores. The results demonstrated that meiotic anomalies functioned as a reproductive barrier occurred before the HS genes acted in gamete of the interspecific hybrid. Although such meiotic anomalies are detrimental to pollen development, the early rescue of microspores carrying the diploid gamete resulted in the fertile regenerated plants. The five partially fertile plants carrying tetraploid genomes with heterozygous alleles of the HS loci produced fertile diploid pollens, implying that the diploid gametes circumvented the allelic conflicts at the HS loci. We also proposed how diploid male gametes avoid HS with the killer-protector model.
Rights: https://creativecommons.org/licenses/by-nc/4.0/
Type: article
URI: http://hdl.handle.net/2115/80153
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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