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Hokkaido University Collection of Scholarly and Academic Papers >
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Genome-wide DNA methylation analysis in canine gastrointestinal lymphoma
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| Title: | Genome-wide DNA methylation analysis in canine gastrointestinal lymphoma |
| Authors: | Ohta, Hiroshi Browse this author | | Yamazaki, Jumpei Browse this author | | Jelinek, Jaroslav Browse this author | | Ishizaki, Teita Browse this author | | Kagawa, Yumiko Browse this author | | Yokoyama, Nozomu Browse this author | | Nagata, Noriyuki Browse this author | | Sasaki, Noboru Browse this author | | Takiguchi, Mitsuyoshi Browse this author →KAKEN DB |
| Keywords: | DNA methylation | | dog | | epigenetics | | gastrointestinal lymphoma |
| Issue Date: | May-2020 |
| Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 82 |
| Issue: | 5 |
| Start Page: | 632 |
| End Page: | 638 |
| Publisher DOI: | 10.1292/jvms.19-0547 |
| Abstract: | DNA methylation is the covalent modification of methyl groups to DNA mostly at CpG dinucleotides and one of the most studied epigenetic mechanisms that leads to gene expression variability without affecting the DNA sequence. Genome-wide analysis of DNA methylation identified the signatures that could define subtypes of human lymphoma patients. The objective of this study was to conduct the genome-wide analysis of DNA methylation in dogs with gastrointestinal lymphoma (GIL). Genomic DNA was extracted from endoscopic biopsies from 10 dogs with GIL. We performed Digital Restriction Enzyme Assay of DNA Methylation (DREAM) for genome-wide DNA methylation analysis that could provide highly quantitative information on DNA methylation levels of CpG sites across the dog genome. We successfully obtained data of quantitative DNA methylation level for 148,601-162,364 CpG sites per GIL sample. Next, we analyzed 83,132 CpG sites to dissect the differences in DNA methylation between GIL and normal peripheral blood mononuclear cells (PBMCs). We found 383-3,054 CpG sites that were hypermethylated in GIL cases compared to PBMCs. Interestingly, 773 CpG sites including promoter regions of 61 genes were identified to be commonly hypermethylated in more than half of the cases, suggesting conserved DNA methylation patterns that are abnormal in GIL. This study revealed that there was a large number of hypermethylated sites that are common in most of canine GIL. These abnormal DNA methylation could be involved in tumorigenesis of the canine GIL. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80176 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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