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Selenium modulates inorganic mercury induced cytotoxicity and intrinsic apoptosis in PC12 cells

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Title: Selenium modulates inorganic mercury induced cytotoxicity and intrinsic apoptosis in PC12 cells
Authors: Hossain, Kaniz Fatima Binte Browse this author
Rahman, Md Mostafizur Browse this author
Sikder, Md Tajuddin Browse this author
Hosokawa, Toshiyuki Browse this author →KAKEN DB
Saito, Takeshi Browse this author →KAKEN DB
Kurasaki, Masaaki Browse this author →KAKEN DB
Keywords: Oxidative stress
Issue Date: 1-Jan-2021
Publisher: Elsevier
Journal Title: Ecotoxicology and environmental safety
Volume: 207
Start Page: 111262
Publisher DOI: 10.1016/j.ecoenv.2020.111262
Abstract: Mercury (Hg) in its all forms, including inorganic Hg (iHg) is an environmental contaminant due to toxicity and diseases in human. However, a little is known about the underlying mechanisms responsible for iHg toxicity. Selenium (Se) is an essential trace element, recognized as an antioxidant and protective agent against metal toxicities. The purpose of this research was to investigate ameliorations of Se counter to iHg-mediated toxicity in PC12 cells. Cytotoxic assays have been shown that iHg (5 mu M) caused oxidative stress and intrinsic apoptosis via ROS generation, oxidizing glutathione, damaging DNA, degrading cell membrane integrity, down-regulating mTOR, p-mTOR, akt and ERK1, and up-regulating cleaved caspase 3 and cytochrome c release in PC12 cells 48 h after incubation. Co-treatment of Se (5 mu M) inhibited intrinsic apoptosis and oxidative stress induced by iHg (5 mu M) via inhibiting ROS formation, boosting GPx contents, increasing reduced glutathione, limiting DNA degradation, improving cell membrane integrity, up-regulating mTOR, p-mTOR, akt, ERK1 and caspase 3, and down-regulating cleaved caspase 3 and cytochrome c leakage in PC12 cells. In conclusion, these results rec-ommended that excessive ROS generation acts a critical role in iHg-influenced oxidative stress and co-treatment of Se attenuates iHg-cytotoxicity through its antioxidant properties.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藏崎 正明

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