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Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration

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Title: Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration
Authors: Yamazaki, Yohei Browse this author
Sakai, Yuichi Browse this author
Yu, Juanwen Browse this author
Mino, Sayaka Browse this author →KAKEN DB
Sawabe, Tomoo Browse this author →KAKEN DB
Keywords: Gut microbiome
Gut regeneration
Sea cucumber
Apostichopus japonicus
Issue Date: 1-Dec-2020
Publisher: PeerJ
Journal Title: Peerj
Volume: 8
Start Page: e10260
Publisher DOI: 10.7717/peerj.10260
Abstract: Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to a lack of experimental models. To track the dynamics of individual gut microbiomes during gut regeneration, both caged mariculture and laboratory isolator systems of sea cucumbers (Apostichopus japonicus) were developed and longitudinal meta16S analyses were performed. Under natural environmental conditions in the caged mariculture system, both bacterial and eukaryotic communities in sea cucumbers' guts appeared to be reconstructed within 4 months after evisceration. Using the laboratory isolator, which can trace daily dynamics, we found that fecal microbiota collected before evisceration were clearly different from those collected after evisceration. We also identified eight key bacteria, belonging to Alteromonadaceae, Rhodobacteraceae, Oceanospirillaceae and family-unassigned Gammaproteobacteria, suggesting that these bacteria might interact with the host during the gut regeneration process. Six of the eight key bacteria were isolated for further bioassay using the isolator developed in this study to test whether these isolates affect gut regeneration.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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