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Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold
Title: | Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold |
Authors: | Kuboki, Yoshinori Browse this author →KAKEN DB | Furusawa, Toshitake Browse this author | Sato, Masaaki Browse this author | Sun, Yongkun Browse this author | Unuma, Hidero Browse this author | Abe, Shigeaki Browse this author →KAKEN DB | Fujisawa, Ryuichi Browse this author | Akasaka, Tsukasa Browse this author →KAKEN DB | Watari, Fumio Browse this author →KAKEN DB | Takita, Hiroko Browse this author →KAKEN DB | Sammons, Rachel Browse this author |
Keywords: | Bone phosphoprotein | titanium | bone formation | chromatography |
Issue Date: | 2014 |
Publisher: | IOS Press |
Journal Title: | Bio-Medical Materials and Engineering |
Volume: | 24 |
Issue: | 3 |
Start Page: | 1539 |
End Page: | 1548 |
Publisher DOI: | 10.3233/BME-140958 |
Abstract: | Based on our previous finding that a chromatography with titanium beads selectively binds phosphoproteins, including caseins, phosvitin and dentin phosphoproteins, we investigated whether bone phosphoproteins also bind to titanium. Bovine bone matrix proteins were extracted with 2 M urea/PBS after demineralization. The 2 M urea extract was directly applied to the titanium chromatography column as reported. The chromatogram showed an initial large peak at breakthrough position (non-binding fraction) and a smaller second peak eluted later (titanium-binding fraction). Both peaks were analyzed by SDS polyacrylamide gel electrophoresis. Stains-all staining which preferentially identifies phospho-proteins revealed that the first peak contained no positively stained band, while the second peak showed 4 or 5 distinctive bands indicative of bone phosphoproteins. To investigate the biological functions of the titanium-binding bone proteins (TiBP), we implanted them into calvaria of rats, combined with titanium web (TW), a highly porous titanium scaffold of thin titanium-fibers. Bone TiBP induced significantly enhanced bone formation, and new bone appeared connected directly to titanium fibers, accompanied by active blood vessel formations. Control TW alone did not induce bone formation within the titanium framework. These results demonstrate that the bone titanium-binding proteins include phosphoproteins which enhance bone formation when implanted into bone with titanium. |
Rights: | The final publication is available at IOS Press through http://dx.doi.org/10.3233/BME-140958 |
Type: | article |
URI: | http://hdl.handle.net/2115/80377 |
Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 久保木 芳徳
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