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Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold

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Title: Bone enhancing effect of titanium-binding proteins isolated from bovine bone and implanted into rat calvaria with titanium scaffold
Authors: Kuboki, Yoshinori Browse this author →KAKEN DB
Furusawa, Toshitake Browse this author
Sato, Masaaki Browse this author
Sun, Yongkun Browse this author
Unuma, Hidero Browse this author
Abe, Shigeaki Browse this author →KAKEN DB
Fujisawa, Ryuichi Browse this author
Akasaka, Tsukasa Browse this author →KAKEN DB
Watari, Fumio Browse this author →KAKEN DB
Takita, Hiroko Browse this author →KAKEN DB
Sammons, Rachel Browse this author
Keywords: Bone phosphoprotein
bone formation
Issue Date: 2014
Publisher: IOS Press
Journal Title: Bio-Medical Materials and Engineering
Volume: 24
Issue: 3
Start Page: 1539
End Page: 1548
Publisher DOI: 10.3233/BME-140958
Abstract: Based on our previous finding that a chromatography with titanium beads selectively binds phosphoproteins, including caseins, phosvitin and dentin phosphoproteins, we investigated whether bone phosphoproteins also bind to titanium. Bovine bone matrix proteins were extracted with 2 M urea/PBS after demineralization. The 2 M urea extract was directly applied to the titanium chromatography column as reported. The chromatogram showed an initial large peak at breakthrough position (non-binding fraction) and a smaller second peak eluted later (titanium-binding fraction). Both peaks were analyzed by SDS polyacrylamide gel electrophoresis. Stains-all staining which preferentially identifies phospho-proteins revealed that the first peak contained no positively stained band, while the second peak showed 4 or 5 distinctive bands indicative of bone phosphoproteins. To investigate the biological functions of the titanium-binding bone proteins (TiBP), we implanted them into calvaria of rats, combined with titanium web (TW), a highly porous titanium scaffold of thin titanium-fibers. Bone TiBP induced significantly enhanced bone formation, and new bone appeared connected directly to titanium fibers, accompanied by active blood vessel formations. Control TW alone did not induce bone formation within the titanium framework. These results demonstrate that the bone titanium-binding proteins include phosphoproteins which enhance bone formation when implanted into bone with titanium.
Rights: The final publication is available at IOS Press through
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保木 芳徳

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