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Design of a liquid cell toward three-dimensional imaging of unidirectionally-aligned particles in solution using X-ray free-electron lasers

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Title: Design of a liquid cell toward three-dimensional imaging of unidirectionally-aligned particles in solution using X-ray free-electron lasers
Authors: Suzuki, Akihiro Browse this author
Kimura, Takashi Browse this author
Yang, Ying Browse this author
Niida, Yoshiya Browse this author
Nishioka, Akiko Browse this author
Tachibana, Tatsuro Browse this author
Takei, Masashi Browse this author
Tono, Kensuke Browse this author
Yabashi, Makina Browse this author
Ishikawa, Tetsuya Browse this author
Oshima, Tairo Browse this author
Bessho, Yoshitaka Browse this author
Joti, Yasumasa Browse this author
Nishino, Yoshinori Browse this author →KAKEN DB
Issue Date: 7-Feb-2020
Publisher: Royal Society of Chemistry
Journal Title: Physical chemistry chemical physics
Volume: 22
Issue: 5
Start Page: 2622
End Page: 2628
Publisher DOI: 10.1039/c9cp03658j
Abstract: X-ray free-electron lasers (XFELs) opened up a possibility for molecular-scale single particle imaging (SPI) without the need for crystallization. In SPI experiments, the orientation of each particle has to be determined from the measured diffraction pattern. Preparing unidirectionally-aligned particles can facilitate the determination of the sample orientation. Here, we show the design principles of a liquid cell for three-dimensional imaging of unidirectionally-aligned particles in solution with XFELs. The liquid cell was designed so that neither incident X-rays nor diffracted X-rays are blocked by the substrate of the liquid cell even at high tilt angles. As a feasibility evaluation, we performed coherent diffraction measurements using the cells with a 1 mm focused XFEL beam. We successfully measured coherent diffraction patterns of a nano-fabricated metal pattern at 701 tilt angle and obtained the reconstructed image by applying iterative phase retrieval. The liquid cell will be usefully applied to molecular-scale SPI by using more tightly focused XFELs. In particular, imaging of membrane proteins embedded in lipid membranes is expected to have an enormous impact on life science and medicine.
Type: article (author version)
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 西野 吉則

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