| Title: | Design of a liquid cell toward three-dimensional imaging of unidirectionally-aligned particles in solution using X-ray free-electron lasers |
| Authors: | Suzuki, Akihiro Browse this author |
| Kimura, Takashi Browse this author |
| Yang, Ying Browse this author |
| Niida, Yoshiya Browse this author |
| Nishioka, Akiko Browse this author |
| Tachibana, Tatsuro Browse this author |
| Takei, Masashi Browse this author |
| Tono, Kensuke Browse this author |
| Yabashi, Makina Browse this author |
| Ishikawa, Tetsuya Browse this author |
| Oshima, Tairo Browse this author |
| Bessho, Yoshitaka Browse this author |
| Joti, Yasumasa Browse this author |
| Nishino, Yoshinori Browse this author →KAKEN DB |
| Issue Date: | 7-Feb-2020 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Physical chemistry chemical physics |
| Volume: | 22 |
| Issue: | 5 |
| Start Page: | 2622 |
| End Page: | 2628 |
| Publisher DOI: | 10.1039/c9cp03658j |
| Abstract: | X-ray free-electron lasers (XFELs) opened up a possibility for molecular-scale single particle imaging (SPI) without the need for crystallization. In SPI experiments, the orientation of each particle has to be determined from the measured diffraction pattern. Preparing unidirectionally-aligned particles can facilitate the determination of the sample orientation. Here, we show the design principles of a liquid cell for three-dimensional imaging of unidirectionally-aligned particles in solution with XFELs. The liquid cell was designed so that neither incident X-rays nor diffracted X-rays are blocked by the substrate of the liquid cell even at high tilt angles. As a feasibility evaluation, we performed coherent diffraction measurements using the cells with a 1 mm focused XFEL beam. We successfully measured coherent diffraction patterns of a nano-fabricated metal pattern at 701 tilt angle and obtained the reconstructed image by applying iterative phase retrieval. The liquid cell will be usefully applied to molecular-scale SPI by using more tightly focused XFELs. In particular, imaging of membrane proteins embedded in lipid membranes is expected to have an enormous impact on life science and medicine. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/80381 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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