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Surface charge dominated protein absorption on hydrogels

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Title: Surface charge dominated protein absorption on hydrogels
Authors: Guo, Honglei Browse this author
Uehara, Yuto Browse this author
Matsuda, Takahiro Browse this author
Kiyama, Ryuji Browse this author
Li, Long Browse this author
Ahmed, Jamil Browse this author
Katsuyama, Yoshinori Browse this author
Nonoyama, Takayuki Browse this author →KAKEN DB
Kurokawa, Takayuki Browse this author →KAKEN DB
Issue Date: 21-Feb-2020
Publisher: Royal Society of Chemistry
Journal Title: Soft Matter
Volume: 16
Issue: 7
Start Page: 1897
End Page: 1907
Publisher DOI: 10.1039/C9SM01999E
Abstract: Candidate materials towards soft tissue engineering require anti-fouling materials that are biocompatible, protein anti-fouling, and mechanically flexible. Conventional hydrogels having more than 70 wt% water are available to design with these criteria in mind. However, some of hydrogels are difficulty to apply hydrogels in internal body organs, because some undesirable proteins absorption on their surfaces. Previously, due to the lack of an effective method in observing the true charge densities of hydrogels,the reason why electrostatic interactions dominate protein absorption behavior is still unclear. In this work, we adopt the microelectrode technique (MET) to study the electrical potential of hydrogels that having negative, positive and neutral potentials,and demonstrate the protein absorption behavior on those hydrogels. The results show that the MET is an effective method to obtain the surface charge densities of various hydrogels. Furthermore, the amount of proteins absorbed to the gel is quantified by the charge density of hydrogels. These results indicate that the electrostatic absorption was quantitatively dominated by a combination of the charge density of hydrogels and the overall charges of proteins. With the knowledge obtained in this work, the effect of surface charges in a hydrogel on protein absorption could be well understood, and this result could be prospective to promote the application of hydrogel in tissue engineering.
Type: article (author version)
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 黒川 孝幸

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