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High mobility of lattice molecules and defects during the early stage of protein crystallization
Title: | High mobility of lattice molecules and defects during the early stage of protein crystallization |
Authors: | Yamazaki, Tomoya Browse this author | Van Driessche, Alexander E. S. Browse this author | Kimura, Yuki Browse this author →KAKEN DB |
Issue Date: | 28-Feb-2020 |
Publisher: | Royal Society of Chemistry |
Journal Title: | Soft matter |
Volume: | 16 |
Issue: | 8 |
Start Page: | 1955 |
End Page: | 1960 |
Publisher DOI: | 10.1039/c9sm02382h |
Abstract: | Protein crystals are expected to be useful not only for their molecular structure analysis but also as functional materials due to their unique properties. Although the generation and the propagation of defects during crystallization play critical roles in the final properties of protein crystals, the dynamics of these processes are poorly understood. By time-resolved liquid-cell transmission electron microscopy, we observed that nanosized crystal defects are surprisingly mobile during the early stages of the crystallization of a lysozyme as a model protein. This highly dynamic behavior of defects reveals that the lattice molecules are mobile throughout the crystal structure. Moreover, the disappearance of the defects indicated that intermolecular bonds can break and reform rapidly with little energetic cost, as reported in theoretical studies. All these findings are in marked contrast to the generally accepted notion that crystal lattices are rigid with very limited mobility of individual lattice molecules. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/80511 |
Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 勇気
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