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Lipidomic profiling of dairy cattle oocytes by high performance liquid chromatography-high resolution tandem mass spectrometry for developmental competence markers

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Title: Lipidomic profiling of dairy cattle oocytes by high performance liquid chromatography-high resolution tandem mass spectrometry for developmental competence markers
Authors: Chen, Zhen Browse this author →KAKEN DB
Wu, Yue Browse this author
Nagano, Masashi Browse this author
Ueshiba, Kouki Browse this author
Furukawa, Eri Browse this author
Yamamoto, Yusuke Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Oocyte
Quantitative lipidomics
Dairy cattle
Energy metabolism
LC/MS
Issue Date: 1-Mar-2020
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 144
Start Page: 56
End Page: 66
Publisher DOI: 10.1016/j.theriogenology.2019.11.039
PMID: 31918070
Abstract: A comparative lipidomic profiling analysis of dairy cattle oocytes with different developmental competences was performed using a combination of high performance liquid chromatography-high resolution tandem mass spectrometry and multivariate statistical analysis. Significant lipidomic changes were identified in degenerating oocytes. Total triacylglycerol in the degenerating oocytes was 1.8-fold higher than that in the normal oocytes; however, total cardiolipin was 53.5% lesser than that in the normal oocytes, which indicated attenuation of energy metabolism. Compared to those in the normal oocytes, triacylglycerols in the degenerating oocytes were composed of longer and more unsaturated acyl chains. In contrast, the acyl chains in free fatty acids present in the degenerating oocytes were shorter and with lesser degree of unsaturation compared to those in the normal oocytes. Moreover, a significant decrease in degenerating oocytes were found in total phosphatidylinositol (14.8 +/- 7.6 pmol vs. 24.8 +/- 5.5 pmol), total phosphatidylcholine (20.8 +/- 8.7 pmol vs. 33.5 +/- 7.2 pmol), and total plasmalogen ethanolamine (9.0 +/- 4.7 pmol vs. 16.8 +/- 5.2 pmol), which indicated dysfunction of lipid-metabolizing enzymes in oocytes during degeneration. Thus, increase of triacylglycerols together with the decrease of certain phospholipid species could be potential markers of oocyte developmental competence. In addition to providing a new approach to investigate the lipidomic changes in oocyte development, the lipidomic profiling in the present study has revealed insights that hold potential to unravel the role of lipid metabolism in oocyte developmental competence in cattle. (C) 2019 Elsevier lnc. All rights reserved.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/80551
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 陳 震

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