| Title: | Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice |
| Authors: | Toraya, Hisashi Browse this author |
| Hasegawa, Tomoka Browse this author →KAKEN DB |
| Sakagami, Naoko Browse this author |
| Tsuchiya, Erika Browse this author |
| Kudo, Ai Browse this author |
| Zhao, Shen Browse this author |
| Moritani, Yasuhito Browse this author |
| Abe, Miki Browse this author |
| Yoshida, Taiji Browse this author |
| Yamamoto, Tomomaya Browse this author |
| Yamamoto, Tsuneyuki Browse this author →KAKEN DB |
| Oda, Kimimitsu Browse this author →KAKEN DB |
| Udagawa, Nobuyuki Browse this author |
| Freitas, PHL. Browse this author |
| Li, Minqi Browse this author →KAKEN DB |
| Amizuka, Norio Browse this author →KAKEN DB |
| Issue Date: | 1-Apr-2017 |
| Publisher: | Biomedical Research Press |
| Journal Title: | Biomedical Research |
| Volume: | 38 |
| Issue: | 2 |
| Start Page: | 123 |
| End Page: | 134 |
| Publisher DOI: | 10.2220/biomedres.38.123 |
| Abstract: | Since osteoblastic activities are believed to be coupled with osteoclasts, we have attempted to histologically verify which of the distinct cellular circumstances, the presence of osteoclasts themselves or bone resorption by osteoclasts, is essential for coupled osteoblastic activity, by examining c-fos−/− or c-src−/− mice. Osteopetrotic c-fos deficient (c-fos−/−) mice have no osteoclasts, while c-src deficient (c-src−/−) mice, another osteopetrotic model, develop dysfunctional osteoclasts due to a lack of ruffled borders. c-fos−/− mice possessed no tartrate-resistant acid phosphatase (TRAPase)-reactive osteoclasts, and showed very weak tissue nonspecific alkaline phosphatase (TNALPase)-reactive mature osteoblasts. In contrast, c-src−/− mice had many TNALPase-positive osteoblasts and TRAPase-reactive osteoclasts. Interestingly, the parallel layers of TRAPase-reactive/osteopontin-positive cement lines were observed in the superficial region of c-src−/− bone matrix. This indicates the possibility that in c-src−/− mice, osteoblasts were activated to deposit new bone matrices on the surfaces that osteoclasts previously passed along, even without bone resorption. Transmission electron microscopy demonstrated cell-to-cell contacts between mature osteoblasts and neighboring ruffled border-less osteoclasts, and osteoid including many mineralized nodules in c-src−/− mice. Thus, it seems likely that osteoblastic activities would be maintained in the presence of osteoclasts, even if they are dysfunctional. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80591 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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