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Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice

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Title: Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
Authors: Toraya, Hisashi Browse this author
Hasegawa, Tomoka Browse this author →KAKEN DB
Sakagami, Naoko Browse this author
Tsuchiya, Erika Browse this author
Kudo, Ai Browse this author
Zhao, Shen Browse this author
Moritani, Yasuhito Browse this author
Abe, Miki Browse this author
Yoshida, Taiji Browse this author
Yamamoto, Tomomaya Browse this author
Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Oda, Kimimitsu Browse this author →KAKEN DB
Udagawa, Nobuyuki Browse this author
Freitas, PHL. Browse this author
Li, Minqi Browse this author →KAKEN DB
Amizuka, Norio Browse this author →KAKEN DB
Issue Date: 1-Apr-2017
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 38
Issue: 2
Start Page: 123
End Page: 134
Publisher DOI: 10.2220/biomedres.38.123
Abstract: Since osteoblastic activities are believed to be coupled with osteoclasts, we have attempted to histologically verify which of the distinct cellular circumstances, the presence of osteoclasts themselves or bone resorption by osteoclasts, is essential for coupled osteoblastic activity, by examining c-fos−/− or c-src−/− mice. Osteopetrotic c-fos deficient (c-fos−/−) mice have no osteoclasts, while c-src deficient (c-src−/−) mice, another osteopetrotic model, develop dysfunctional osteoclasts due to a lack of ruffled borders. c-fos−/− mice possessed no tartrate-resistant acid phosphatase (TRAPase)-reactive osteoclasts, and showed very weak tissue nonspecific alkaline phosphatase (TNALPase)-reactive mature osteoblasts. In contrast, c-src−/− mice had many TNALPase-positive osteoblasts and TRAPase-reactive osteoclasts. Interestingly, the parallel layers of TRAPase-reactive/osteopontin-positive cement lines were observed in the superficial region of c-src−/− bone matrix. This indicates the possibility that in c-src−/− mice, osteoblasts were activated to deposit new bone matrices on the surfaces that osteoclasts previously passed along, even without bone resorption. Transmission electron microscopy demonstrated cell-to-cell contacts between mature osteoblasts and neighboring ruffled border-less osteoclasts, and osteoid including many mineralized nodules in c-src−/− mice. Thus, it seems likely that osteoblastic activities would be maintained in the presence of osteoclasts, even if they are dysfunctional.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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