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Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus

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Title: Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus
Authors: Kishimoto, Mai Browse this author
Hang'ombe, Bernard M. Browse this author
Hall, William W. Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Keywords: Encephalomyocarditis virus
Mastomys natalensis
Issue Date: 3-Feb-2021
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 102
Issue: 3
Start Page: 001564
Publisher DOI: 10.1099/jgv.0.001564
PMID: 33533710
Abstract: Encephalomyocarditis virus (EMCV) infects a wide range of hosts and can cause encephalitis, myocarditis, reproductive disorders and diabetes mellitus in selected mammalian species. As for humans, EMCV infection seems to occur by the contact with animals and can cause febrile illnesses in some infected patients. Here we isolated EMCV strain ZM12/14 from a natal multimammate mouse (Mastomys natalensis: M. natalensis) in Zambia. Pairwise sequence similarity of the ZM12/14 P1 region consisting of antigenic capsid proteins showed the highest similarity of nucleotide (80.7 %) and amino acid (96.2%) sequence with EMCV serotype 1 (EMCV-1). Phylogenetic analysis revealed that ZM12/14 clustered into EMCV-1 at the P1 and P3 regions but segregated from known EMCV strains at the P2 region, suggesting a unique evolutionary history. Reverse transcription PCR (RT-PCR) screening and neutralizing antibody assays for EMCV were performed using collected tissues and serum from various rodents (n=179) captured in different areas in Zambia. We detected the EMCV genome in 19 M. natalensis (19/179=10.6 %) and neutralizing antibody for EMCV in 33 M. natalensis (33/179=18.4 %). However, we did not detect either the genome or neutralizing antibody in other rodent species. High neutralizing antibody litres (≧320) were observed in both RT-PCR-negative and -positive animals. Inoculation of ZM12/14 caused asymptomatic persistent infection in BALB/c mice with high antibody titres and high viral loads in some organs, consistent with the above epidemiological results. This study is the first report of the isolation of EMCV in Zambia, suggesting that M. natalensis may play a role as a natural reservoir of infection.
Rights: © Mai Kishimoto, Bernard M. Hang'ombe, William W. Hall, Yasuko Orba, Hirofumi Sawa and Michihito Sasaki, 2021. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, volume102, issue3, 2021, 10.1099/jgv.0.001564.
Type: article (author version)
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸本 麻衣

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