| Title: | E190V substitution of H6 hemagglutinin is one of key factors for binding to sulfated sialylated glycan receptor and infection to chickens |
| Authors: | Kikutani, Yuto1 Browse this author |
| Okamatsu, Masatoshi2 Browse this author →KAKEN DB |
| Nishihara, Shoko3 Browse this author |
| Takase, Sayaka4 Browse this author →KAKEN DB |
| Hiono, Takahiro5 Browse this author →KAKEN DB |
| de Vries, P. Robert6 Browse this author |
| McBride, Ryan7 Browse this author |
| Matsuno, Keita8 Browse this author →KAKEN DB |
| Kida, Hiroshi9 Browse this author →KAKEN DB |
| Sakoda, Yoshihiro10 Browse this author →KAKEN DB |
| Authors(alt): | Yoden, Sayaka4 |
| Keywords: | avian influenza virus |
| hemagglutinin |
| interspecies transmission |
| sialic acid receptor |
| sulfated glycans |
| Issue Date: | 16-Apr-2020 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Microbiology and immunology |
| Volume: | 64 |
| Issue: | 4 |
| Start Page: | 304 |
| End Page: | 312 |
| Publisher DOI: | 10.1111/1348-0421.12773 |
| PMID: | 31943329 |
| Abstract: | Avian influenza viruses (AIVs) recognize sialic acid linked alpha 2,3 to galactose (SA alpha 2,3Gal) glycans as receptors. In this study, the interactions between hemagglutinins (HAs) of AIVs and sulfated SA alpha 2,3Gal glycans were analyzed to clarify the molecular basis of interspecies transmission of AIVs from ducks to chickens. It was revealed that E190V and N192D substitutions of the HA increased the recovery of viruses derived from an H6 duck virus isolate, A/duck/Hong Kong/960/1980 (H6N2), in chickens. Recombinant HAs from an H6 chicken virus, A/chicken/Tainan/V156/1999 (H6N1), bound to sulfated SA alpha 2,3Gal glycans, whereas the HAs from an H6 duck virus did not. Binding preference of mutant HAs revealed that an E190V substitution is critical for the recognition of sulfated SA alpha 2,3Gal glycans. These results suggest that the binding of the HA from H6 AIVs to sulfated SA alpha 2,3Gal glycans explains a part of mechanisms of interspecies transmission of AIVs from ducks to chickens. |
| Rights: | This is the peer reviewed version of the following article: Kikutani Y, OkamatsuM, Nishihara S, et al. E190V substitution of H6hemagglutinin is one of key factors for binding tosulfated sialylated glycan receptor and infection tochickens.Microbiology and Immunology. 2020;1–9, which has been published in final form at https://doi.org/10.1111/1348-0421.12773. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/81008 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
