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Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-gamma by a novel stem cell niche tracking method

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Title: Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-gamma by a novel stem cell niche tracking method
Authors: Yokoi, Yuki Browse this author
Adachi, Takahiro Browse this author
Sugimoto, Rina Browse this author
Kikuchi, Mani Browse this author
Ayabe, Tokiyoshi Browse this author
Nakamura, Kiminori Browse this author →KAKEN DB
Keywords: Paneth cell
Intestinal stem cell
Stem cell niche
Interferon-gamma
Live imaging
Issue Date: 19-Mar-2021
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 545
Start Page: 14
End Page: 19
Publisher DOI: 10.1016/j.bbrc.2021.01.050
Abstract: Paneth cells and Lgr5+ intestinal stem cells (Lgr5+ ISCs) constitute the stem cell niche and maintain small intestinal epithelial integrity by recognizing various niche factors derived from subepithelial cells and external antigens. Although it has been known that interferon-gamma (IFN-gamma), a Th1 cytokine, is associated with intestinal epithelial disruption during inflammation as a niche factor, dynamics of Paneth cells and Lgr5+ ISCs in response to IFN-gamma remain to be understood. Here we show that CAG-tdTomato;Lgr5-EGFP (CT-LE) mice generated in this study enable to identify Paneth cells and Lgr5+ ISCs separately by fluorescence signals. Lgr5+ ISCs underwent cell death a little earlier than Paneth cells in response to IFN-gamma by simultaneous tracking using CT-LE mice. In addition, the timing of cell death in most Paneth cells overlapped with Lgr5+ ISCs, suggesting that Paneth cell depletion is induced directly by IFN-gamma. Taken together, we established a novel simultaneous stem cell niche tracking method and clarified the involvement of both Paneth cells and Lgr5+ ISCs in stem cell niche damage induced by IFN-gamma, further contribute to understanding the mechanism for maintaining intestinal homeostasis by stem cell niche. (C) 2021 The Authors. Published by Elsevier Inc.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/81037
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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