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Opposing functions of alpha- and beta-adrenoceptors in the formation of processes by cultured astrocytes
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Title: | Opposing functions of alpha- and beta-adrenoceptors in the formation of processes by cultured astrocytes |
Authors: | Kitano, Taisuke Browse this author | Eguchi, Ryota Browse this author →KAKEN DB | Okamatsu-Ogura, Yuko Browse this author →KAKEN DB | Yamaguchi, Soichiro Browse this author →KAKEN DB | Otsuguro, Ken-ichi Browse this author →KAKEN DB |
Keywords: | Astrocyte | Process formation | Noradrenaline | Adrenoceptor | Cyclic AMP |
Issue Date: | Mar-2021 |
Publisher: | Japanese Pharmacological Society |
Journal Title: | Journal of pharmacological sciences |
Volume: | 145 |
Issue: | 3 |
Start Page: | 228 |
End Page: | 240 |
Publisher DOI: | 10.1016/j.jphs.2020.12.005 |
Abstract: | Astrocytes are glial cells with numerous fine processes which are important for the functions of the central nervous system. The activation of beta-adrenoceptors induces process formation of astrocytes via cyclic AMP (cAMP) signaling. However, the role of alpha-adrenoceptors in the astrocyte morphology has not been elucidated. Here, we examined it by using cultured astrocytes from neonatal rat spinal cords and cortices. Exposure of these cells to noradrenaline and the beta-adrenoceptor agonist isoproterenol increased intracellular cAMP levels and induced the formation of processes. Noradrenaline-induced process formation was enhanced with the alpha(1)-adrenoceptor antagonist prazosin and alpha(2)-adrenoceptor antagonist atipamezole. Atipamezole also enhanced noradrenaline-induced cAMP elevation. Isoproterenol-induced process formation was not inhibited by the alpha(1)-adrenoceptor agonist phenylephrine but was inhibited by the alpha(2)-adrenoceptor agonist dexmedetomidine. Dexmedetomidine also inhibited process formation induced by the adenylate cyclase activator forskolin and the membrane-permeable cAMP analog dibutyryl-cAMP. Moreover, dexmedetomidine inhibited cAMP-independent process formation induced by adenosine or the Rho-associated kinase inhibitor Y27632. In the presence of propranolol, noradrenaline inhibited Y27632-induced process formation, which was abolished by prazosin or atipamezole. These results demonstrate that alpha-adrenoceptors inhibit both cAMP-dependent and -independent astrocytic process formation. (C) 2020 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. |
Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/81065 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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