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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
| Authors: | Murata, Shiro Browse this author →KAKEN DB | | Taniguchi, Ayaka Browse this author | | Isezaki, Masayoshi Browse this author | | Fujisawa, Sotaro Browse this author | | Sakai, Eishi Browse this author | | Taneno, Akira Browse this author | | Ichii, Osamu Browse this author →KAKEN DB | | Ito, Takuya Browse this author | | Maekawa, Naoya Browse this author →KAKEN DB | | Okagawa, Tomohiro Browse this author →KAKEN DB | | Konnai, Satoru Browse this author →KAKEN DB | | Ohashi, Kazuhiko Browse this author →KAKEN DB |
| Keywords: | Poultry red mite | | Cysteine protease | | Cathepsin L | | Vaccine candidate | | Deg-CPR-1 | | Dermanyssus gallinae |
| Issue Date: | 3-Feb-2021 |
| Publisher: | EDP Sciences |
| Journal Title: | Parasite |
| Volume: | 28 |
| Start Page: | 9 |
| Publisher DOI: | 10.1051/parasite/2021005 |
| Abstract: | Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy. |
| Rights: | https://creativecommons.org/licenses/by/4.0 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81083 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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