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Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens

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Title: Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens
Authors: Murata, Shiro Browse this author →KAKEN DB
Taniguchi, Ayaka Browse this author
Isezaki, Masayoshi Browse this author
Fujisawa, Sotaro Browse this author
Sakai, Eishi Browse this author
Taneno, Akira Browse this author
Ichii, Osamu Browse this author →KAKEN DB
Ito, Takuya Browse this author
Maekawa, Naoya Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author →KAKEN DB
Konnai, Satoru Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Poultry red mite
Cysteine protease
Cathepsin L
Vaccine candidate
Dermanyssus gallinae
Issue Date: 3-Feb-2021
Publisher: EDP Sciences
Journal Title: Parasite
Volume: 28
Start Page: 9
Publisher DOI: 10.1051/parasite/2021005
Abstract: Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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